释放愈合潜力:探索用于烧伤伤口恢复的下一代再生蛋白质纳米支架

IF 10.7 1区 医学 Q1 PHARMACOLOGY & PHARMACY Asian Journal of Pharmaceutical Sciences Pub Date : 2023-11-01 DOI:10.1016/j.ajps.2023.100856
Liangwei Si , Xiong Guo , Hriday Bera , Yang Chen , Fangfang Xiu , Peixin Liu , Chunwei Zhao , Yasir Faraz Abbasi , Xing Tang , Vito Foderà , Dongmei Cun , Mingshi Yang
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摘要

烧伤是一个严重的公共卫生问题,科学家们一直致力于开发有前途的仿生敷料,以有效地管理烧伤创面。在本研究中,我们建立了基于α-乳清蛋白(ALA)的静电纺纳米纤维支架(ENs)与其他再生蛋白支架相比,在烧伤创面愈合中的更大功效及其相关机制。将牛血清白蛋白(BSA)、ⅰ型胶原蛋白(COL)、溶菌酶(LZM)和ALA分别与聚ε-己内酯(PCL)共混制得4种不同的复合ENs (LZM/PCL、BSA/PCL、COL/PCL和ALA/PCL ENs)。亲水性复合支架表现出增强的润湿性和可变的力学性能。ALA/PCL ENs比其他复合材料ENs具有更高的成纤维细胞增殖和粘附水平,与PCL ENs和其他复合材料支架相比,ALA/PCL ENs还能促进再生皮肤组织的成熟,并在三度烧伤模型上表现出与胶原海绵Ⓡ相当的伤口愈合效果。与其他ENs相比,ALA/PCL ENs的伤口愈合活性增强可能归因于它们促进伤口部位血清素产生的能力。总的来说,这项研究表明,与其他再生蛋白相比,ALA是一种独特的蛋白质,当负载在纳米纤维支架中时,它具有更大的烧伤伤口愈合潜力。
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Unleashing the healing potential: Exploring next-generation regenerative protein nanoscaffolds for burn wound recovery

Burn injury is a serious public health problem and scientists are continuously aiming to develop promising biomimetic dressings for effective burn wound management. In this study, a greater efficacy in burn wound healing and the associated mechanisms of α-lactalbumin (ALA) based electrospun nanofibrous scaffolds (ENs) as compared to other regenerative protein scaffolds were established. Bovine serum albumin (BSA), collagen type I (COL), lysozyme (LZM) and ALA were separately blended with poly(ε-caprolactone) (PCL) to fabricate four different composite ENs (LZM/PCL, BSA/PCL, COL/PCL and ALA/PCL ENs). The hydrophilic composite scaffolds exhibited an enhanced wettability and variable mechanical properties. The ALA/PCL ENs demonstrated higher levels of fibroblast proliferation and adhesion than the other composite ENs. As compared to PCL ENs and other composite scaffolds, the ALA/PCL ENs also promoted a better maturity of the regenerative skin tissues and showed a comparable wound healing effect to Collagen sponge on third-degree burn model. The enhanced wound healing activity of ALA/PCL ENs compared to other ENs could be attributed to their ability to promote serotonin production at wound sites. Collectively, this investigation demonstrated that ALA is a unique protein with a greater potential for burn wound healing as compared to other regenerative proteins when loaded in the nanofibrous scaffolds.

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来源期刊
Asian Journal of Pharmaceutical Sciences
Asian Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
18.30
自引率
2.90%
发文量
11
审稿时长
14 days
期刊介绍: The Asian Journal of Pharmaceutical Sciences (AJPS) serves as the official journal of the Asian Federation for Pharmaceutical Sciences (AFPS). Recognized by the Science Citation Index Expanded (SCIE), AJPS offers a platform for the reporting of advancements, production methodologies, technologies, initiatives, and the practical application of scientific knowledge in the field of pharmaceutics. The journal covers a wide range of topics including but not limited to controlled drug release systems, drug targeting, physical pharmacy, pharmacodynamics, pharmacokinetics, pharmacogenomics, biopharmaceutics, drug and prodrug design, pharmaceutical analysis, drug stability, quality control, pharmaceutical engineering, and material sciences.
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