含kartogenin的丝素-硫酸软骨素混合水凝胶:控释研究及生物活性

IF 2.5 4区 医学 Q4 MATERIALS SCIENCE, BIOMATERIALS International Journal of Polymeric Materials and Polymeric Biomaterials Pub Date : 2023-09-22 DOI:10.1080/00914037.2023.2255716
Maryam Farokhi, Atefeh Solouk, Hamid Mirzadeh, Heinz Redl, Andreas Teuschl-Woller
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引用次数: 0

摘要

摘要在丝素/硫酸软骨素(SF) /硫酸软骨素(CS)混合溶液中,通过诱导二酪氨酸键,采用酶交联的方法合成了含有kartogenin (KGN)的丝素-硫酸软骨素-杂化水凝胶(HHGs)。然后进行物理交联,优化HHG的性能。结果表明,物理交联后β-片含量从10%增加到40%,对KGN累积释放率影响近3倍。利用C28/I2人软骨细胞系进行的生物学研究表明,KGN、CS和优化后的β-sheets含量可改善HHG的细胞支持行为。最后,KGN负载的HHG可能是软骨修复的有希望的候选者。酶法交联和物理交联法制备丝素和硫酸软骨素双交联杂交水凝胶的示意图。酶交联:在丝素酚基团之间形成二酪氨酸形成键,在丝素β-片之间物理锁定硫酸软骨素。与水和/或乙醇处理的物理交联最终会增加β-片的含量。作者要感谢Saeed Bahrami博士和Davood Sadeghi博士在分析生物学结果方面的帮助。作者要感谢Amirkabir理工大学(德黑兰理工学院)专业实验室和技术服务办公室对这项研究的支持,资助号为1401-0506。作者贡献:smaryam Farokhi:概念化,方法论,验证,形式分析,调查,写作-原始草案,写作-审查和编辑,以及可视化。Atefeh Solouk:概念,方法,资源,写作-审查和编辑,监督,资金获取和项目管理。Hamid Mirzadeh:概念化、方法论、资源、监督、项目管理和资金获取。Andreas Teuschl-Woller:概念化,监督,方法论,资源,写作-审查和编辑。Heinz Redl:概念、方法、资源、监督和项目管理。伦理批准:由于这是一项体外研究,因此本研究不需要伦理声明。披露声明本研究不存在利益冲突。数据可用性声明不适用。作者要感谢Amirkabir理工大学(德黑兰理工大学)专业实验室和技术服务办公室对这项研究的支持,资助号为1401-0506。
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Kartogenin-loaded silk fibroin–chondroitin sulfate hybrid hydrogel with tailored β-sheet content: control release studies and biological activity
AbstractSilk fibroin (SF)–chondroitin sulfate (CS)-hybrid hydrogels (HHGs) containing kartogenin (KGN) were synthesized by the induction of di-tyrosine bonds, using enzymatic crosslinking in SF/CS blend solution. Then, physical crosslinking was performed to optimize the HHG properties. The results showed that the increasing of the β-sheet content as side effect of physical crosslinking from 10% to 40% affects KGN accumulative release percentage almost three times. Biological investigations using C28/I2 human chondrocyte cell line showed that the presence of KGN, CS, and optimized β-sheets content improved cell supporting behavior of HHG. Finally, KGN loaded HHG might be a promising candidate for cartilage repair.Schematic representation of fabrication of the dual crosslinked hybrid hydrogel containing silk fibroin and chondroitin sulfate by enzymatic crosslinking and physical crosslinking method for kartogenin release. Enzymatic crosslinking: shaping di-tyrosine formation bond between silk fibroin phenol groups and locking physically chondroitin sulfate between silk fibroin β-sheets. Physical crosslinking with water and/or ethanol treatments end up in increased β-sheets content.Keywords: β-sheetkartogeninphysical crosslinkingrelease kineticssilk fibroin AcknowledgementsThe authors would like to thank Dr. Saeed Bahrami and Dr. Davood Sadeghi for their assistance in analyzing the biological results. The authors would like to express their gratitude to the office of Professional Laboratories and Technology Services in Amirkabir University of Technology (Tehran Polytechnic) for supporting this research under Grant Number 1401-0506.Author contributionsMaryam Farokhi: conceptualization, methodology, validation, formal analysis, investigation, writing – original draft, writing – review and editing, and visualization. Atefeh Solouk: conceptualization, methodology, resources, writing – review and editing, supervision, funding acquisition, and project administration. Hamid Mirzadeh: conceptualization, methodology, resources, supervision, project administration, and funding acquisition. Andreas Teuschl-Woller: conceptualization, supervision, methodology, resources, and writing-review and editing. Heinz Redl: conceptualization, methodology, resources, supervision, and project administration.Ethical approvalSince this is an in vitro study, no ethical declaration is required for this study.Disclosure statementThere is no conflict of interest associated with this research.Data availability statementNot applicable.Additional informationFundingThe authors would like to express their gratitude to the Office of Professional Laboratories and Technology Services in Amirkabir University of Technology (Tehran Polytechnic) for supporting this research under Grant Number 1401-0506.
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来源期刊
International Journal of Polymeric Materials and Polymeric Biomaterials
International Journal of Polymeric Materials and Polymeric Biomaterials Chemical Engineering-General Chemical Engineering
CiteScore
8.00
自引率
3.10%
发文量
97
审稿时长
3.3 months
期刊介绍: International Journal of Polymeric Materials and Polymeric Biomaterials is the official publication of the International Society for Biomedical Polymers and Polymeric Biomaterials (ISBPPB). This journal provides a forum for the publication of peer-reviewed, English language articles and select reviews on all aspects of polymeric materials and biomedical polymers. Being interdisciplinary in nature, this journal publishes extensive contributions in the areas of encapsulation and controlled release technologies to address innovation needs as well.
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