Michaela S. Matthes, Norman B. Best, Janlo M. Robil, Paula McSteen
{"title":"生长素水平降低对缺硼玉米突变体tassel - less1发育缺陷的影响","authors":"Michaela S. Matthes, Norman B. Best, Janlo M. Robil, Paula McSteen","doi":"10.1002/jpln.202300155","DOIUrl":null,"url":null,"abstract":"Abstract Background Plant responses to deficiencies of the micronutrient boron are diverse and go beyond the well‐characterized function of boron in cell wall crosslinking. To explain these phenotypic discrepancies, hypotheses about interactions of boron with various phytohormones have been proposed, particularly auxin. While these hypotheses are intensely tested in the root meristem of the model species, Arabidopsis thaliana , studies in crop species and the shoot are limited. Aims To address potential boron–auxin interactions during the vegetative and reproductive development of the crop maize ( Zea mays ), we utilized the boron‐deficient tassel‐less1 ( tls1 ) mutant and the auxin‐deficient vanishing tassel2 ( vt2 ) mutant. We investigated interactions of boron and auxin on the levels of auxin biosynthesis and auxin transport in leaves and shoot meristems. Methods and Results By using genetic interaction analysis, hormone quantification, and confocal microscopy, we show that boron‐deficient leaf phenotypes in tls1 are enhanced in double mutants with vt2 in both greenhouse and field conditions. However, auxin levels are not altered in developing leaves in tls1 . Rather, the localization of Zm PIN1a:YFP, a marker for auxin transport, is altered in young tassel meristems and is absent from organ initiation sites during vegetative development. Conclusions Our data suggest a link between polar auxin transport and phenotypic consequences in boron‐deficient conditions and further show that boron deficiency‐induced developmental defects are sensitive to low auxin levels. Our study, therefore, offers new insight into nutrient–hormone interactions to regulate crop development.","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Enhancement of developmental defects in the boron‐deficient maize mutant <i>tassel‐less1</i> by reduced auxin levels\",\"authors\":\"Michaela S. Matthes, Norman B. Best, Janlo M. Robil, Paula McSteen\",\"doi\":\"10.1002/jpln.202300155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Background Plant responses to deficiencies of the micronutrient boron are diverse and go beyond the well‐characterized function of boron in cell wall crosslinking. To explain these phenotypic discrepancies, hypotheses about interactions of boron with various phytohormones have been proposed, particularly auxin. While these hypotheses are intensely tested in the root meristem of the model species, Arabidopsis thaliana , studies in crop species and the shoot are limited. Aims To address potential boron–auxin interactions during the vegetative and reproductive development of the crop maize ( Zea mays ), we utilized the boron‐deficient tassel‐less1 ( tls1 ) mutant and the auxin‐deficient vanishing tassel2 ( vt2 ) mutant. We investigated interactions of boron and auxin on the levels of auxin biosynthesis and auxin transport in leaves and shoot meristems. Methods and Results By using genetic interaction analysis, hormone quantification, and confocal microscopy, we show that boron‐deficient leaf phenotypes in tls1 are enhanced in double mutants with vt2 in both greenhouse and field conditions. However, auxin levels are not altered in developing leaves in tls1 . Rather, the localization of Zm PIN1a:YFP, a marker for auxin transport, is altered in young tassel meristems and is absent from organ initiation sites during vegetative development. Conclusions Our data suggest a link between polar auxin transport and phenotypic consequences in boron‐deficient conditions and further show that boron deficiency‐induced developmental defects are sensitive to low auxin levels. Our study, therefore, offers new insight into nutrient–hormone interactions to regulate crop development.\",\"PeriodicalId\":16802,\"journal\":{\"name\":\"Journal of Plant Nutrition and Soil Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-09-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Plant Nutrition and Soil Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/jpln.202300155\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Nutrition and Soil Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jpln.202300155","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Enhancement of developmental defects in the boron‐deficient maize mutant tassel‐less1 by reduced auxin levels
Abstract Background Plant responses to deficiencies of the micronutrient boron are diverse and go beyond the well‐characterized function of boron in cell wall crosslinking. To explain these phenotypic discrepancies, hypotheses about interactions of boron with various phytohormones have been proposed, particularly auxin. While these hypotheses are intensely tested in the root meristem of the model species, Arabidopsis thaliana , studies in crop species and the shoot are limited. Aims To address potential boron–auxin interactions during the vegetative and reproductive development of the crop maize ( Zea mays ), we utilized the boron‐deficient tassel‐less1 ( tls1 ) mutant and the auxin‐deficient vanishing tassel2 ( vt2 ) mutant. We investigated interactions of boron and auxin on the levels of auxin biosynthesis and auxin transport in leaves and shoot meristems. Methods and Results By using genetic interaction analysis, hormone quantification, and confocal microscopy, we show that boron‐deficient leaf phenotypes in tls1 are enhanced in double mutants with vt2 in both greenhouse and field conditions. However, auxin levels are not altered in developing leaves in tls1 . Rather, the localization of Zm PIN1a:YFP, a marker for auxin transport, is altered in young tassel meristems and is absent from organ initiation sites during vegetative development. Conclusions Our data suggest a link between polar auxin transport and phenotypic consequences in boron‐deficient conditions and further show that boron deficiency‐induced developmental defects are sensitive to low auxin levels. Our study, therefore, offers new insight into nutrient–hormone interactions to regulate crop development.
期刊介绍:
Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years.
Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH.
Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are:
JPNSS – Topical Divisions
Special timely focus in interdisciplinarity:
- sustainability & critical zone science.
Soil-Plant Interactions:
- rhizosphere science & soil ecology
- pollutant cycling & plant-soil protection
- land use & climate change.
Soil Science:
- soil chemistry & soil physics
- soil biology & biogeochemistry
- soil genesis & mineralogy.
Plant Nutrition:
- plant nutritional physiology
- nutrient dynamics & soil fertility
- ecophysiological aspects of plant nutrition.