{"title":"用de81阴离子交换纸固相化学降解法同时测序RNA","authors":"Burckhard Gross, André Rosenthal","doi":"10.1016/0735-0651(87)90019-7","DOIUrl":null,"url":null,"abstract":"<div><p>A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3′-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"4 3","pages":"Pages 57-61"},"PeriodicalIF":0.0000,"publicationDate":"1987-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(87)90019-7","citationCount":"4","resultStr":"{\"title\":\"Simultaneous sequencing of RNA by solid-phase chemical degradation using DE 81 anion-exchange paper\",\"authors\":\"Burckhard Gross, André Rosenthal\",\"doi\":\"10.1016/0735-0651(87)90019-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3′-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"4 3\",\"pages\":\"Pages 57-61\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1987-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(87)90019-7\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0735065187900197\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0735065187900197","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
以Whatman DE 81阴离子交换纸为载体，建立了一种同时测序RNA和RNA片段的固相化学降解方法。该方法包括以下操作:(1)将3 '端标记的RNA或RNA片段固定在DE 81纸上;(2)洗涤;(3)改性反应;(4)清洗;(5)对纸段进行分类;(6)苯胺反应;(7)冻干法;(8) TEAB解吸RNA;(9)冻干。
Simultaneous sequencing of RNA by solid-phase chemical degradation using DE 81 anion-exchange paper
A solid-phase chemical degradation method for simultaneous sequencing of RNA and RNA fragments has been developed using Whatman DE 81 anion-exchange paper as the support. The approach involves the following operations: (1) immobilization of the 3′-end labeled RNAs or RNA fragments on DE 81 paper; (2) washing; (3) modification reactions; (4) washing; (5) sorting of the paper segments; (6) aniline reaction; (7) lyophilization; (8) desorption of the RNA by TEAB; and (9) lyophilization.
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