原生芽孢杆菌分离株单独及联合防治秋粘虫不同幼虫的效果

IF 2.1 3区 农林科学 Q1 ENTOMOLOGY Egyptian Journal of Biological Pest Control Pub Date : 2023-10-11 DOI:10.1186/s41938-023-00743-7
J. Karshanal, Vinay Kumari Kalia
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引用次数: 0

摘要

摘要背景落粘虫(FAW)是鳞翅目:夜蛾科的一种入侵性多食害虫,对多种农作物造成严重危害。由于它们的喂养方式和抗药性的发展,农药的使用受到限制。因此,本研究旨在确定昆虫病原菌(芽孢杆菌)在致病性和生长抑制方面对FAW的致病性。在这项研究中,最初从印度不同栖息地分离的49株芽孢杆菌分离株以及5株参考菌株,在控制的实验室条件下对其对frugiperda新生儿的效力进行了筛选,然后进行了毒力和组合生物测定。结果5株本土分离芽孢杆菌(VKK1、VKK5、S16C2、S25C1和SOIL 20)在单一浓度(1000 μg−1日粮)下对frugiperda新生儿的死亡率为35.49% ~ 65.52%。对这5株分离菌株和1株参考菌株Btk -HD1(苏云金芽孢杆菌血清库斯塔克菌株HD1)进行进一步检测,以确定对frugiperda新生儿的中位致死浓度(LC 50)。其中,本地菌株VKK5的LC 50最低(718.40µg/g), HD1的LC 50最高(3352µg/g)。VKK5和VKK1组合对雏虫和3龄幼虫具有加性效应。此外,还记录了生长抑制。结论Bt菌株联用可在发育初期增强对FAW幼虫的致病性,在后期影响其生长发育。因此,利用昆虫病原菌(Bt)对飞蛾进行生物防治对飞蛾的有效防治具有重要意义。
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Efficacy of native Bacillus isolates against different larval instars of fall armyworm, Spodoptera frugiperda alone and in combination
Abstract Background Fall armyworm (FAW) Spodoptera frugiperda (J.E. Smith) Lepidoptera: Noctuidae is an invasive polyphagous pest that causes severe damage to several agricultural crops. The use of pesticides is limited because of their mode of feeding and resistant development. Hence, the present work aimed to determine the pathogenicity of entomopathogenic bacteria ( Bacillus spp.) against FAW in terms of mortality and growth inhibition. In this study, initially 49 native Bacillus isolates, isolated from diverse habitats in India, along with five reference strains, were screened for their efficacy against neonates of S. frugiperda under controlled laboratory conditions, followed by virulence and combinatorial bioassays. Results Five native Bacillus isolates (VKK1, VKK5, S16C2, S25C1, and SOIL 20) showed mortality in the range of 35.49–65.52% against neonates of S. frugiperda at single concentration (1000 μg g −1 of diet). These five isolates, along with one reference strain Btk -HD1 ( Bacillus thuringiensis serovar kurstaki strain HD1), were further tested to find the median lethal concentration (LC 50 ) for neonates of S. frugiperda . Among these, native Bt strain VKK5 showed the lowest LC 50 (718.40 µg/g of diet) and HD1 showed the highest LC 50 (3352 µg/g of diet). Combinatorial bioassay against neonate and third instar larvae showed that the combination of VKK5 and VKK1 had an additive effect. Moreover, growth inhibition was also recorded. Conclusion The combination of Bt strains leads to an enhancement of pathogenicity toward FAW larvae at the initial stage of development, and in later stages, it affects their growth and development. Thus, biocontrol of FAW by entomopathogenic bacteria ( Bt ) can play a vital role in the effective management of FAW.
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来源期刊
CiteScore
4.90
自引率
16.70%
发文量
128
审稿时长
>12 weeks
期刊介绍: The Egyptian Journal of Biological Pest Control is a periodic scientific journal published by the Egyptian Society for Biological Control of Pests (ESBCP) in collaboration with SpringerNature. The journal aims to publish internationally peer-reviewed, high-quality research articles in the field of biological and integrated pest control (non-chemical control). The journal publishes review articles, original papers, conference reports, book reviews, editorials, laboratory reports, technical notes and short communications.
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