基于3D生物打印的单肝肿瘤球体分析黄曲霉毒素b1诱导的耐药癌细胞

IF 6.8 3区 医学 Q1 ENGINEERING, BIOMEDICAL International Journal of Bioprinting Pub Date : 2023-08-18 DOI:10.36922/ijb.0985
Viet Phuong Cao, Sera Hong, Joon Myong Song
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引用次数: 0

摘要

黄曲霉毒素B1存在于多种食物中,是一种已知会致癌的霉菌毒素。因此,人类可能会通过日常饮食接触到它。本研究通过生物3D打印技术建立了一个三维(3D)肿瘤球体模型,以检验HepG2肝肿瘤球体暴露于黄曲霉毒素B1是否会增加单个肿瘤球体中耐药肝癌细胞的数量。两种生物标志物CD133 (pronin -1)和醛脱氢酶1 (ALDH1)被用来鉴定在单个肝肿瘤球体中形成的耐药癌细胞。通过单球体成像和荧光激活细胞分选(FACS)检测单个肿瘤球体对耐药癌细胞的诱导作用。利用两种方法在单个肿瘤球体水平上定量监测黄曲霉毒素B1引起的耐药癌细胞的增加,其呈剂量依赖性。生物3D打印制备单个肝癌球体模型成功测定黄曲霉毒素B1致肝癌耐药细胞
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3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell
Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1
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来源期刊
CiteScore
6.90
自引率
4.80%
发文量
81
期刊介绍: The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.
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