{"title":"基于3D生物打印的单肝肿瘤球体分析黄曲霉毒素b1诱导的耐药癌细胞","authors":"Viet Phuong Cao, Sera Hong, Joon Myong Song","doi":"10.36922/ijb.0985","DOIUrl":null,"url":null,"abstract":"Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1","PeriodicalId":48522,"journal":{"name":"International Journal of Bioprinting","volume":null,"pages":null},"PeriodicalIF":6.8000,"publicationDate":"2023-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell\",\"authors\":\"Viet Phuong Cao, Sera Hong, Joon Myong Song\",\"doi\":\"10.36922/ijb.0985\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1\",\"PeriodicalId\":48522,\"journal\":{\"name\":\"International Journal of Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.8000,\"publicationDate\":\"2023-08-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36922/ijb.0985\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36922/ijb.0985","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
3D bioprinting-based single liver tumor spheroid analysis for aflatoxin B1-induced drug-resistant cancer cell
Aflatoxin B1, found in a variety of foods, is a mycotoxin known to cause cancer. Therefore, humans may be exposed to it through their daily diet. In this study, a three-dimensional (3D) tumor spheroid model was developed via 3D bioprinting to examine whether exposure of HepG2 liver tumor spheroids to aflatoxin B1 can increase the population of drug-resistant liver cancer cells in a single tumor spheroid. Two biomarkers, CD133 (prominin-1) and aldehyde dehydrogenase 1 (ALDH1), were used to identify drug-resistant cancer cells formed in the single liver tumor spheroids. The induction of drug-resistant cancer cells in the single tumor spheroids was examined through single spheroid imaging and fluorescence-activated cell sorting (FACS). The increase of drug-resistant cancer cells, which was caused by aflatoxin B1 in a dose-dependent manner, was quantitatively monitored at the single tumor spheroid level using both methods. 3D bioprinting-fabricated single liver tumor spheroid model successfully determined drug-resistant liver cancer cells caused by aflatoxin B1
期刊介绍:
The International Journal of Bioprinting is a globally recognized publication that focuses on the advancements, scientific discoveries, and practical implementations of Bioprinting. Bioprinting, in simple terms, involves the utilization of 3D printing technology and materials that contain living cells or biological components to fabricate tissues or other biotechnological products. Our journal encompasses interdisciplinary research that spans across technology, science, and clinical applications within the expansive realm of Bioprinting.