用氯化金染色昆虫发育中的和成体的神经肌肉连接处。

M B Rheuben, P J Schaner
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引用次数: 2

摘要

单个昆虫的肌肉纤维,其神经肌肉连接处已经用一种改进的兰维尔氯化金方法染色,如果在肌肉上加上醛,就可以自由地解剖和安装整个肌肉。沿着单个纤维的长度的神经肌肉连接被很好地描绘出来,并且可以被测量和计数。有效的方法包括用柠檬酸钠缓冲至低pH的戊二醛固定,或在中性pH下将戊二醛和多聚甲醛混合在磷酸盐缓冲液中固定,然后暴露于柠檬酸和氯化金。由于节肢动物的神经末梢不释放乙酰胆碱作为一种递质,而且不能被更常用的胆碱酯酶染色,因此该方法很方便,可用于一般节肢动物神经肌肉连接的研究。
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The use of gold chloride to stain developing and adult neuromuscular junctions in the insect.

Individual insect muscle fibers, whose neuromuscular junctions have been stained with a modification of Ranvier's gold chloride method, can be dissected free and mounted whole if the muscle is prefixed in aldehydes. The neuromuscular junctions along the length of the individual fibers are well delineated and can be measured and counted. Effective procedures include fixation with glutaraldehyde buffered to low pH with sodium citrate, or glutaraldehyde and paraformaldehyde combined in phosphate buffer at neutral pH, followed by exposure to citric acid and to gold chloride. The method is convenient, and could be useful for the study of arthropod neuromuscular junctions in general, since their nerve terminals do not release acetylcholine as a transmitter and cannot be stained by the more commonly used cholinesterase methods.

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