经周期酸处理后使用硫代氨基脲可使肾小球基底膜甲基胺银染色更快、更干净。

I Hayashi, Y Tome, Y Shimosato
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引用次数: 14

摘要

常用于肾小球基底膜显示的周期性酸-甲基苯丙胺银染色技术对技术要求较高,在某些情况下可能难以获得良好的结果。为了克服这种困难和不一致性,我们改进了方法,用周期酸氧化后进行甲基苯丙胺银染色,然后应用硫代氨基脲。在此过程中,氨基脲增强了甲基胺银与肾小球基底膜的反应,与常规方法相比,反应在更短的时间内完成。这种修饰还消除了与载玻片表面和溶液容器的任何非特异性反应,并且无论采用何种固定方法和材料,都产生了极好的可重复性结果。在家兔肾小球基底膜上也发现了染色,这是常规方法难以证实的。
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Thiosemicarbazide used after periodic acid makes methenamine silver staining of renal glomerular basement membranes faster and cleaner.

The periodic acid-methenamine silver staining technique, which is frequently used for demonstrating the renal glomerular basement membrane, requires a high degree of skill, and in some cases it may be difficult to obtain a good result. To overcome such difficulty and inconsistency, we have improved the method by performing methenamine silver staining after oxidation with periodic acid and subsequent application of thiosemicarbazide. In this procedure, this semicarbazide enhanced the reaction of methenamine silver with the glomerular basement membrane and the reaction was completed within a shorter time in comparison with the conventional method. This modification also eliminated any nonspecific reaction with the surface of the glass slide and the solution container and yielded excellent and reproducible results irrespective of the fixation method and material employed. It was also found to stain the renal glomerular basement membrane of rabbits, which is demonstrable only with difficulty by the conventional method.

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