基于CFD软件的疟疾检测微流控PCR芯片装置设计

IF 1.9 Q2 MATHEMATICS, INTERDISCIPLINARY APPLICATIONS Computation Pub Date : 2023-09-30 DOI:10.3390/computation11100190
Meynard Austria, Jon Patrick Garcia, Alvin Caparanga, Lemmuel Tayo, Bonifacio Doma
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引用次数: 0

摘要

聚合酶链反应(PCR)技术是检测疟疾的DNA扩增分子方法之一。然而,通过传统PCR收集和运输样品以及处理和传播结果,特别是用于常规临床实践时,可能会妨碍该技术的敏感性和特异性。由于医疗设备供应有限和国家经济状况不佳,这种疾病在菲律宾的猖獗加剧了;因此,有必要创新一种早期检测疟疾的设备。因此,本研究的重点是设计一种微流控装置,该装置将模仿基于18S rRNA基因的传统属特异性PCR的功能,用于检测低级别寄生虫血症的疟原虫(恶性疟原虫)。设计的目的是便携、方便和经济,这是过去的文献中没有专门针对疟疾检测的。这是国内第一个专门为此设计的硅设计。利用ANSYS计算流体动力学(CFD)分析软件对该装置进行了研制和仿真。仿真结果表明,在设计中加入环路会增加其相对偏差,但与仅采用直线路径设计相比,其相对偏差最小。这表明在设计微流控装置以最小化芯片长度时,环路是可以接受的。研究还发现,增大流路截面积会降低设计效率。最后,在三种材料中,聚丙烯制成的切屑效率最高,相对偏差为0.94,而聚碳酸酯和聚二甲基硅氧烷的相对偏差分别为2.78和1.92。由于本研究中使用的软件的局限性,未来的研究人员可能会对44循环的微流控芯片进行网格化,并可能评估其他材料,如生物复合材料,以扩大设计的应用范围。
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Designing Microfluidic PCR Chip Device Using CFD Software for the Detection of Malaria
Polymerase chain reaction (PCR) technique is one of the molecular methods in amplifying DNA for the detection of malaria. However, the collection and transportation of samples and the processing and dissemination of results via conventional PCR, especially when used for routine clinical practice, can hamper the technique’s sensitivity and specificity. The rampancy of such disease in the Philippines is aggravated by the limited supply of medical machinery and the poor economic state of the country; thus, the need to innovate a device for the early detection of malaria is necessary. With that, this study focuses on designing a microfluidic device that will mimic the function of a conventional genus-specific PCR based on the 18S rRNA gene to detect malaria parasites (Plasmodium falciparum) at low-grade parasitemia. The design was intended to be portable, accessible, and economical, which none from past literature has dealt with specifically for malaria detection. This in silico design is a first in the country specially crafted for such reasons. The proposed device was developed and simulated using ANSYS software for Computational Fluid Dynamics (CFD) analyses. The simulation shows that adding loops to the design increases its relative deviation but minimally compared to having only a straight path design. This indicates that looping is acceptable in designing a microfluidic device to minimize chip length. It was also found that increasing the cross-sectional area of the fluid path decreases the efficiency of the design. Lastly, among the three materials utilized, the chip made of polypropylene is the most efficient, with a relative deviation of 0.94 compared to polycarbonate and polydimethylsiloxane, which have relative deviations of 2.78 and 1.92, respectively. Future researchers may mesh the 44-cycle microfluidic chip due to the limitations of the software used in this study, and other materials, such as biocomposites, may be assessed to broaden the application of the design.
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来源期刊
Computation
Computation Mathematics-Applied Mathematics
CiteScore
3.50
自引率
4.50%
发文量
201
审稿时长
8 weeks
期刊介绍: Computation a journal of computational science and engineering. Topics: computational biology, including, but not limited to: bioinformatics mathematical modeling, simulation and prediction of nucleic acid (DNA/RNA) and protein sequences, structure and functions mathematical modeling of pathways and genetic interactions neuroscience computation including neural modeling, brain theory and neural networks computational chemistry, including, but not limited to: new theories and methodology including their applications in molecular dynamics computation of electronic structure density functional theory designing and characterization of materials with computation method computation in engineering, including, but not limited to: new theories, methodology and the application of computational fluid dynamics (CFD) optimisation techniques and/or application of optimisation to multidisciplinary systems system identification and reduced order modelling of engineering systems parallel algorithms and high performance computing in engineering.
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