封闭根系沼泽蔓越莓离体生长完成特性研究

I. V. Nechiporenko, S. V. Akimova, P. O. Kazakov
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引用次数: 0

摘要

及时性。沼泽蔓越莓(Vaccinium oxycoccos L.)工业化种植的先决条件,导致对高质量的克隆繁殖(离体)种植材料的需求增加。在温室条件下,对不同矿质肥料和不同光照对蔓越莓植株离体生长完成度的影响进行了研究。方法。以克隆微繁技术获得的蔓越莓(Vaccinium oxycoccos L.)的离体植株为研究对象。试验适应的蔓越莓植株种植在0.5 L容器中,在酸度至少为pH 3.5-4.0的‘Veltorf’泥炭基质中,根据不同品种添加矿质肥料:APAVIVA N15P15K15(S10) 0.08和0.16 g/L,磺胺磷N16P20(S12) 0.072和0.144 g/L, N12P52 0.1和0.2 g/L,对照不施肥。这些植物被放置在不同类型的光下:在LED植物灯(UnionPowerStar - 40W-T)下,光周期为16小时,在温室条件下的自然光下(没有使用额外的照明)。结果。结果表明,在沼泽蔓越莓(Vaccinium oxycoccos L.)盆栽离体生长完成过程中,LED植物灯的优势得到了体现,在生长第42天,在所有实验变异体中使用LED植物灯,植株发育的形态测量指标几乎是自然光下植株的2倍。矿肥类型和光照类型对芽长(82.9±13.74 ~ 107.4±35.95 cm,对照58.6±20.92 cm)和叶表面积(41.1±6.46 ~ 54.1±4.67 cm2,对照22.9±9.63 cm2)影响显著。结论。我们获得的结果有助于更好地代表沼泽蔓越莓(Vaccinium oxycoccos L.)植物在不同类型的照明(16小时光周期的植物照明和自然光-不添加额外光)和矿物质营养最佳剂量的选择下的离体生长条件。在LED照明下,N16P20(S12)浓度为0.072 g/L, N15P15K15(S10)浓度为0.16 g/L。
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Peculiarities of ex vitro growing completion of bog cranberry (Vaccinium oxycoccos L.) with a closed root system
Timeliness. The prerequisites for the cultivation of bog cranberry ( Vaccinium oxycoccos L.) in industrial plantations have led to an increased demand for high quality planting material obtained by clonal micropropagation ( in vitro ). Studies have been carried out on the of ex vitro growing completion of cranberry plants using different mineral fertilizers and types of light under greenhouse conditions. Methods. Ex vitro plants of a selected form of cranberry ( Vaccinium oxycoccos L.) obtained using the clonal micropropagation technique were the objects of research. Experimental adapted cranberry plants were planted in 0.5 L containers in 'Veltorf' peat substrate with an acidity of at least pH 3.5-4.0, to which mineral fertilizers were added according to the variants: APAVIVA N15P15K15(S10) 0.08 and 0.16 g/L, Sulfoammophos N16P20(S12) 0.072 and 0.144 g/L, N12P52 0.1 and 0.2 g/L, control without fertilizer. The plants were placed under different types of light: under LED phytolamps (UnionPowerStar – 40W-T) with a photoperiod of 16 hours and under natural light (without the use of additional lighting) under greenhouse conditions. Results . It was found that during of the ex vitro growing completion of bog cranberry ( Vaccinium oxycoccos L.) plants in containers, the advantage of LED phytolamps was revealed, when using them in all experimental variants on the 42nd day of growing the morphometric indicators of plant development were almost 2 times higher than in plants grown under natural light. The type of mineral fertilizers and type light significantly influenced the total length of shoots (82.9±13.74- 107.4±35.95 cm vs. 58.6±20.92 cm in control) and leaf surface area (41.1±6.46-54.1±4.67 cm2 vs. 22.9±9.63 cm2 ). Conclusion. The results we obtained contributed to a better representation of the ex vitro growing conditions of bog cranberry ( Vaccinium oxycoccos L.) plants using different types of lighting (phyto-lighting with a 16-hour photoperiod and natural light – without adding additional light) and the selection of optimal doses of mineral nutrition. The best, under LED lighting, were N16P20(S12) at a concentration of 0.072 g/L and N15P15K15(S10) at a concentration of 0.16 g/L.
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