滑液PCR分析对犬莱姆病的诊断价值

O. Panteleienko, T. Tsarenko
{"title":"滑液PCR分析对犬莱姆病的诊断价值","authors":"O. Panteleienko, T. Tsarenko","doi":"10.33245/2310-4902-2023-180-1-59-69","DOIUrl":null,"url":null,"abstract":"Lyme borreliosis, also known as Lyme disease, is a chronic multiorgan disease of humans and animals transmitted by ticks of the Ixodidae family and caused by a group of spirochetes Borrelia burgdorferi sensu lato. Dogs are one of the most susceptible animal species to Lyme disease. Symptoms of the disease in dogs can range from mild lameness and fever to more severe and potentially life-threatening conditions, such as kidney damage and neurological disorders. Due to the variety of clinical manifestations and the lack of a universal diagnostic approach, veterinarians often face difficulties in accurately diagnosing the disease. Therefore, improving the algorithms for diagnosing Lyme borreliosis in dogs remains an urgent issue for practical veterinary medicine. The article describes and analyzes data on two cases of dogs from Kyiv (Ukraine) that presented with musculoskeletal disorders, including lameness, swelling of the knee joints, and tenderness, which raised suspicion of Lyme borreliosis. The diagnostic tests used to confirm the diagnosis of Lyme borreliosis included clinical blood test, C-reactive protein test, cerebrospinal fluid cytology, enzyme-linked immunosorbent assay for IgM and IgG antigens to Borrelia burgdorferi sensu lato, computed tomography and PCR analysis of synovial fluid for the presence of Borrelia burgdorferi sensu lato DNA. As a result, the clinical blood test, C-reactive protein test, and cerebrospinal fluid cytology were not diagnostically important for the diagnosis of Lyme borreliosis. In both cases, serological tests for antibodies to Borrelia burgdorferi sensu lato were not positive. Computed tomography showed the development of an inflammatory process in the knee joints with the formation of osteophytes, enthesophytes, sclerosis of the articular surfaces, and an increase in the volume of synovial bursa. The final diagnosis of Lyme arthritis in both dogs was established by PCR analysis of synovial fluid from the affected joints for the presence of Borrelia burgdorferi sensu lato DNA. This study emphasizes the importance of considering Lyme borreliosis as a potential cause of musculoskeletal disorders in dogs. We also propose an expanded algorithm for the diagnosis of Lyme borreliosis in dogs with a pronounced symptom complex of Lyme arthritis using the method of PCR analysis of synovial fluid to detect the DNA of Borrelia burgdorferi sensu lato spirochetes. Key words: Lyme borreliosis in dogs, clinical cases, diagnosis, polymerase chain reaction, Borrelia burgdorferi sensu lato.","PeriodicalId":18841,"journal":{"name":"Naukovij vìsnik veterinarnoï medicini","volume":"6 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Diagnostic value of PCR analysis of synovial fluid for the diagnosis of Lyme borreliosis in dogs\",\"authors\":\"O. Panteleienko, T. Tsarenko\",\"doi\":\"10.33245/2310-4902-2023-180-1-59-69\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Lyme borreliosis, also known as Lyme disease, is a chronic multiorgan disease of humans and animals transmitted by ticks of the Ixodidae family and caused by a group of spirochetes Borrelia burgdorferi sensu lato. Dogs are one of the most susceptible animal species to Lyme disease. Symptoms of the disease in dogs can range from mild lameness and fever to more severe and potentially life-threatening conditions, such as kidney damage and neurological disorders. Due to the variety of clinical manifestations and the lack of a universal diagnostic approach, veterinarians often face difficulties in accurately diagnosing the disease. Therefore, improving the algorithms for diagnosing Lyme borreliosis in dogs remains an urgent issue for practical veterinary medicine. The article describes and analyzes data on two cases of dogs from Kyiv (Ukraine) that presented with musculoskeletal disorders, including lameness, swelling of the knee joints, and tenderness, which raised suspicion of Lyme borreliosis. The diagnostic tests used to confirm the diagnosis of Lyme borreliosis included clinical blood test, C-reactive protein test, cerebrospinal fluid cytology, enzyme-linked immunosorbent assay for IgM and IgG antigens to Borrelia burgdorferi sensu lato, computed tomography and PCR analysis of synovial fluid for the presence of Borrelia burgdorferi sensu lato DNA. As a result, the clinical blood test, C-reactive protein test, and cerebrospinal fluid cytology were not diagnostically important for the diagnosis of Lyme borreliosis. In both cases, serological tests for antibodies to Borrelia burgdorferi sensu lato were not positive. Computed tomography showed the development of an inflammatory process in the knee joints with the formation of osteophytes, enthesophytes, sclerosis of the articular surfaces, and an increase in the volume of synovial bursa. The final diagnosis of Lyme arthritis in both dogs was established by PCR analysis of synovial fluid from the affected joints for the presence of Borrelia burgdorferi sensu lato DNA. This study emphasizes the importance of considering Lyme borreliosis as a potential cause of musculoskeletal disorders in dogs. We also propose an expanded algorithm for the diagnosis of Lyme borreliosis in dogs with a pronounced symptom complex of Lyme arthritis using the method of PCR analysis of synovial fluid to detect the DNA of Borrelia burgdorferi sensu lato spirochetes. Key words: Lyme borreliosis in dogs, clinical cases, diagnosis, polymerase chain reaction, Borrelia burgdorferi sensu lato.\",\"PeriodicalId\":18841,\"journal\":{\"name\":\"Naukovij vìsnik veterinarnoï medicini\",\"volume\":\"6 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Naukovij vìsnik veterinarnoï medicini\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33245/2310-4902-2023-180-1-59-69\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Naukovij vìsnik veterinarnoï medicini","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33245/2310-4902-2023-180-1-59-69","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

莱姆病,又称莱姆病,是一种人类和动物的慢性多器官疾病,由伊蚊科蜱虫传播,由一组感氏伯氏疏螺旋体引起。狗是最容易感染莱姆病的动物之一。狗的症状可以从轻微的跛行和发烧到更严重的、可能危及生命的疾病,如肾脏损伤和神经系统疾病。由于临床表现的多样性和缺乏通用的诊断方法,兽医经常面临准确诊断疾病的困难。因此,改进犬莱姆病的诊断算法仍然是兽医实践中亟待解决的问题。这篇文章描述并分析了来自乌克兰基辅的两例狗的数据,这两例狗表现出肌肉骨骼疾病,包括跛行、膝关节肿胀和压痛,这引起了莱姆病的怀疑。用于确诊莱姆病的诊断试验包括临床血检、c反应蛋白检测、脑脊液细胞学检查、伯氏疏螺旋体IgM和IgG抗原酶联免疫吸附试验、计算机断层扫描和滑液PCR检测伯氏疏螺旋体DNA。因此,临床血液检查、c反应蛋白检查和脑脊液细胞学检查对莱姆病的诊断不具有重要的诊断意义。在这两种情况下,伯氏疏螺旋体抗体的血清学测试均未呈阳性。计算机断层扫描显示膝关节炎症过程的发展,伴随骨赘、内生菌的形成,关节表面硬化,滑膜囊体积增加。两只狗的莱姆病的最终诊断是通过对受影响关节的滑液进行PCR分析,以确定是否存在伯氏疏螺旋体的DNA。这项研究强调了考虑莱姆病作为狗肌肉骨骼疾病的潜在原因的重要性。我们还提出了一种扩展算法,用于莱姆病症状复杂的狗的莱姆病诊断,利用滑液PCR分析的方法检测伯氏疏螺旋体的DNA。关键词:犬莱姆病,临床病例,诊断,聚合酶链反应,感伯氏疏螺旋体
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Diagnostic value of PCR analysis of synovial fluid for the diagnosis of Lyme borreliosis in dogs
Lyme borreliosis, also known as Lyme disease, is a chronic multiorgan disease of humans and animals transmitted by ticks of the Ixodidae family and caused by a group of spirochetes Borrelia burgdorferi sensu lato. Dogs are one of the most susceptible animal species to Lyme disease. Symptoms of the disease in dogs can range from mild lameness and fever to more severe and potentially life-threatening conditions, such as kidney damage and neurological disorders. Due to the variety of clinical manifestations and the lack of a universal diagnostic approach, veterinarians often face difficulties in accurately diagnosing the disease. Therefore, improving the algorithms for diagnosing Lyme borreliosis in dogs remains an urgent issue for practical veterinary medicine. The article describes and analyzes data on two cases of dogs from Kyiv (Ukraine) that presented with musculoskeletal disorders, including lameness, swelling of the knee joints, and tenderness, which raised suspicion of Lyme borreliosis. The diagnostic tests used to confirm the diagnosis of Lyme borreliosis included clinical blood test, C-reactive protein test, cerebrospinal fluid cytology, enzyme-linked immunosorbent assay for IgM and IgG antigens to Borrelia burgdorferi sensu lato, computed tomography and PCR analysis of synovial fluid for the presence of Borrelia burgdorferi sensu lato DNA. As a result, the clinical blood test, C-reactive protein test, and cerebrospinal fluid cytology were not diagnostically important for the diagnosis of Lyme borreliosis. In both cases, serological tests for antibodies to Borrelia burgdorferi sensu lato were not positive. Computed tomography showed the development of an inflammatory process in the knee joints with the formation of osteophytes, enthesophytes, sclerosis of the articular surfaces, and an increase in the volume of synovial bursa. The final diagnosis of Lyme arthritis in both dogs was established by PCR analysis of synovial fluid from the affected joints for the presence of Borrelia burgdorferi sensu lato DNA. This study emphasizes the importance of considering Lyme borreliosis as a potential cause of musculoskeletal disorders in dogs. We also propose an expanded algorithm for the diagnosis of Lyme borreliosis in dogs with a pronounced symptom complex of Lyme arthritis using the method of PCR analysis of synovial fluid to detect the DNA of Borrelia burgdorferi sensu lato spirochetes. Key words: Lyme borreliosis in dogs, clinical cases, diagnosis, polymerase chain reaction, Borrelia burgdorferi sensu lato.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Toxicological characteristics of the probiotic drug Bioseven Rationale and efficacy of sexual desire stimulation in mares under anaphrodisia Clinical-radiological, hematological and biochemical assessment of bone replacement with germanium-doped calcium-phosphate ceramics for fragment fractures of tubular bones in dogs Actual problems of the quality and safety of milk and dairy products Influence of antibiotic treatment duration on the development of antibiotic resistance
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1