压缩时间和变形相关的闭合力丧失对大鼠夹致脊髓损伤的影响

IF 3.2 Q2 CLINICAL NEUROLOGY Neurology International Pub Date : 2023-11-13 DOI:10.3390/neurolint15040088
Po-Hsuan Lee, Heng-Juei Hsu, Chih-Hao Tien, Chi-Chen Huang, Chih-Yuan Huang, Hui-Fang Chen, Ming-Long Yeh, Jung-Shun Lee
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引用次数: 0

摘要

脊髓损伤大鼠模型是脊髓损伤临床前研究的关键。然而,文献显示压缩持续时间的可变性和对夹子变形相关的闭合力损失的有限关注。我们的目的是研究压缩时间对脊髓损伤严重程度的影响以及夹片变形对闭合力的影响。大鼠分别接受1、5、10、20和30 s的T10水平夹致脊髓损伤,另一组进行T10横断。结果包括功能、组织学、电生理评估和炎症细胞因子分析。触觉压力映射系统量化开合循环后夹子闭合力。我们的研究结果显示,受压时间与功能、组织学和电生理缺陷的严重程度呈正相关。值得注意的是,即使是短暂的1-s压缩也会导致与中重度脊髓损伤相当的严重缺陷。持续时间超过20秒的SSEP波形被取消。经过5次开合循环后,夹子闭合力减小。这项研究为大鼠模型中调节脊髓损伤严重程度提供了重要的见解,有助于研究人员。了解压缩持续时间和夹疲劳是必不可少的实验设计和解释使用夹诱导SCI模型。
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Characterizing the Impact of Compression Duration and Deformation-Related Loss of Closure Force on Clip-Induced Spinal Cord Injury in Rats
The clip-induced spinal cord injury (SCI) rat model is pivotal in preclinical SCI research. However, the literature exhibits variability in compression duration and limited attention to clip deformation-related loss of closure force. We aimed to investigate the impact of compression duration on SCI severity and the influence of clip deformation on closure force. Rats received T10-level clip-induced SCI with durations of 1, 5, 10, 20, and 30 s, and a separate group underwent T10 transection. Outcomes included functional, histological, electrophysiological assessments, and inflammatory cytokine analysis. A tactile pressure mapping system quantified clip closure force after open–close cycles. Our results showed a positive correlation between compression duration and the severity of functional, histological, and electrophysiological deficits. Remarkably, even a brief 1-s compression caused significant deficits comparable to moderate-to-severe SCI. SSEP waveforms were abolished with durations over 20 s. Decreased clip closure force appeared after five open–close cycles. This study offers critical insights into regulating SCI severity in rat models, aiding researchers. Understanding compression duration and clip fatigue is essential for experiment design and interpretation using the clip-induced SCI model.
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来源期刊
Neurology International
Neurology International CLINICAL NEUROLOGY-
CiteScore
3.70
自引率
3.30%
发文量
69
审稿时长
11 weeks
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