用HPTLC定量评价伞形棉在乳腺癌中的抗氧化和抗癌活性及羽衣酚和β -谷甾醇的含量

Q2 Pharmacology, Toxicology and Pharmaceutics Current Bioactive Compounds Pub Date : 2023-10-10 DOI:10.2174/0115734072268707230921102959

Shilpee Chaudhary, Chandrashekar Kodangala Subraya, Sreedhara Ranganath Karkala, Vasudev Pai, Aswatha Ram Holavanna Halli Nanjundaiah, Aravinda Pai

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引用次数: 0

摘要

简介:漆膜兰(Memecylon umbellatum)是一种多年生植物，在西高止山脉大量生长，传统上用于治疗癌症和炎症。背景:漆膜兰是一种多年生植物，在西高止山脉大量生长，传统上被用于治疗癌症和炎症。方法:从文献查阅中发现，利用HPTLC法鉴定黄皮草的生物活性成分及筛选黄皮草的抗氧化和抗癌活性尚无实质性的工作。为此，采用HPTLC法对其进行植物化学研究，并采用ABTS法、DPPH法、一氧化氮抗氧化法、铁螯合活性法和总抗氧化活性法筛选其抗氧化活性。目的:从文献综述中发现，利用HPTLC法鉴定黄皮草的活性成分及筛选黄皮草的抗氧化和抗癌活性尚无实质性的研究。为此，本文采用HPTLC法对其进行了植物化学研究，并对其抗氧化活性和抗癌活性进行了筛选。用MCF-7和MDA-MB-231细胞体外检测抗肿瘤活性。细胞周期分析表明乙酸乙酯提取物引起MCF-7细胞G0/G1期阻滞。HPTLC分析结果表明，从乙酸乙酯提取物中鉴定出芦皮醇。结论:伞花乙酸乙酯提取物具有明显的抗肿瘤活性。其抗氧化和抗癌活性可能与Lupeol、酚类化合物和黄酮类化合物有关。结论:芦皮醇、酚类化合物和黄酮类化合物可能具有抗氧化和抗癌作用。

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Evaluation of Antioxidant and Anticancer Activities of Memecylon umbellatum in Breast Carcinoma and Lupeol and Beta-sitosterol Quantified by HPTLC

Introduction: Memecylon umbellatum is a perennial plant of the Melastomataceae family that grows in abundance in the Western Ghats and has been used traditionally to treat cancer and inflammatory conditions. background: Memecylon umbellatum is a perennial plant of the Melastomataceae family grows in abundance in the Western Ghats and has been used traditionally to treat cancer and inflammatory conditions. Methods: From the literature survey, it was found that no substantial work has been carried out to identify the bioactive compounds by HPTLC method and to screen the antioxidant and anticancer activity of M. umbellatum. Hence, an effort has been made to carry out the phytochemical investigation by HPTLC method and to screen the antioxidant activity by ABTS assay, DPPH assay, Nitric oxide antioxidant assay, Iron chelating activity and Total antioxidant activity. objective: From the literature survey it was found that no substantial work has been carried out to identify the bioactive compounds by HPTLC method and to screen the antioxidant and anticancer activity of M. umbellatum. Hence an effort has been made to carry out the phytochemical investigation by HPTLC method and to screen the antioxidant activity and anticancer activity Results: Total phenolics and flavonoids were carried out by using suitable methods. MCF-7 as well as MDA-MB-231 cells were used to test anticancer activities in vitro. Cell cycle analysis demonstrated that ethyl acetate extract caused G0/G1 phase arrest in MCF-7 cells. HPTLC analysis led to the identification of Lupeol from ethyl acetate extract. Conclusion: The anticancer activity of ethyl acetate extract of M. umbellatum was significant. The antioxidant and anticancer activity may attributed to Lupeol, Phenolic compounds and Flavonoids. conclusion: The antioxidant and anticancer activity may attributed to Lupeol, Phenolic compounds and Flavonoids.

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来源期刊

Current Bioactive Compounds Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.90

自引率

0.00%

发文量

112

期刊介绍： The journal aims to provide comprehensive review articles on new bioactive compounds with proven activities in various biological screenings and pharmacological models with a special emphasis on stereoeselective synthesis. The aim is to provide a valuable information source of bioactive compounds synthesized or isolated, which can be used for further development of pharmaceuticals by industry and academia. The journal should prove to be essential reading for pharmacologists, natural product chemists and medicinal chemists who wish to be kept informed and up-to-date with the most important developments on new bioactive compounds of natural or synthetic origin, including their stereoeselective synthesis.