食品级二氧化钛和氧化锌纳米颗粒在大鼠口服暴露后诱导毒性和心脏损伤。

IF 7.2 1区 医学 Q1 TOXICOLOGY Particle and Fibre Toxicology Pub Date : 2023-11-17 DOI:10.1186/s12989-023-00553-7
Manuel Alejandro Herrera-Rodríguez, María Del Pilar Ramos-Godinez, Agustina Cano-Martínez, Francisco Correa Segura, Angélica Ruiz-Ramírez, Natalia Pavón, Elizabeth Lira-Silva, Rocío Bautista-Pérez, Rosina Sánchez Thomas, Norma Laura Delgado-Buenrostro, Yolanda Irasema Chirino, Rebeca López-Marure
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引用次数: 0

摘要

背景:金属纳米颗粒(NPs)被广泛用作人类食用的食品添加剂。NPs由于体积小而进入血液,与身体的所有器官和细胞接触。NPs对呼吸道和肠道有不良影响;然而,很少有研究关注口服金属NPs对心血管系统的毒性后果。本文分析了两种食品级添加剂对心血管系统的影响。方法:用E171标记的二氧化钛和氧化锌NPs,每隔一天以10 mg/kg bw的剂量通过食管插管给药Wistar大鼠,连续90 d。我们评估了心肌细胞形态和死亡、心肌线粒体中凋亡和自噬蛋白的表达、线粒体功能障碍和心脏组织中金属的浓度。结果:暴露于E171和ZnO NPs的大鼠心脏组织学表现出重要的形态学变化,如细胞浸润、胶原沉积和线粒体改变。细胞内Cyt-C水平下降,TUNEL阳性细胞增多。炎性细胞因子的表达未见明显变化。两种NPs都改变了线粒体功能,表明心脏功能障碍,这与钙浓度升高有关。ZnO NPs诱导caspases 3和caspases 9以及LC3B和beclin-1两种自噬蛋白的表达,且效果较E171最强。结论:E171和ZnO NPs在暴露90天后会对大鼠的心血管产生不良影响,因此应考虑摄入含有这些添加剂的食物,因为它们会转移到血液中并引起心血管损伤。
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Food-grade titanium dioxide and zinc oxide nanoparticles induce toxicity and cardiac damage after oral exposure in rats.

Background: Metallic nanoparticles (NPs) are widely used as food additives for human consumption. NPs reach the bloodstream given their small size, getting in contact with all body organs and cells. NPs have adverse effects on the respiratory and intestinal tract; however, few studies have focused on the toxic consequences of orally ingested metallic NPs on the cardiovascular system. Here, the effects of two food-grade additives on the cardiovascular system were analyzed.

Methods: Titanium dioxide labeled as E171 and zinc oxide (ZnO) NPs were orally administered to Wistar rats using an esophageal cannula at 10 mg/kg bw every other day for 90 days. We evaluated cardiac cell morphology and death, expression of apoptotic and autophagic proteins in cardiac mitochondria, mitochondrial dysfunction, and concentration of metals on cardiac tissue.

Results: Heart histology showed important morphological changes such as presence of cellular infiltrates, collagen deposition and mitochondrial alterations in hearts from rats exposed to E171 and ZnO NPs. Intracellular Cyt-C levels dropped, while TUNEL positive cells increased. No significant changes in the expression of inflammatory cytokines were detected. Both NPs altered mitochondrial function indicating cardiac dysfunction, which was associated with an elevated concentration of calcium. ZnO NPs induced expression of caspases 3 and 9 and two autophagic proteins, LC3B and beclin-1, and had the strongest effect compared to E171.

Conclusions: E171 and ZnO NPs induce adverse cardiovascular effects in rats after 90 days of exposure, thus food intake containing these additives, should be taken into consideration, since they translocate into the bloodstream and cause cardiovascular damage.

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来源期刊
CiteScore
15.90
自引率
4.00%
发文量
69
审稿时长
6 months
期刊介绍: Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.
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