Imran Ali Khan , Vasundhara Kamineni , Subhadra Poornima , Parveen Jahan , Qurratulain Hasan , Pragna Rao
{"title":"孕妇肿瘤坏死因子α启动子多态性研究","authors":"Imran Ali Khan , Vasundhara Kamineni , Subhadra Poornima , Parveen Jahan , Qurratulain Hasan , Pragna Rao","doi":"10.1016/j.jrhm.2015.01.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Aims</h3><p>The aim of this study was to explore the possible association between the −850<!--> <span><span><span>C/T polymorphism in the tumor necrosis factor alpha (TNF-α) gene promoter, and pregnancy-associated diseases such as </span>gestational diabetes mellitus (GDM) and </span>preeclampsia<span> (PE), in south Indian women. GDM and PE are common complications that occur during pregnancy and are the leading causes of perinatal mortality. To date, the mechanisms that initiate GDM and PE in humans have remained elusive.</span></span></p></div><div><h3>Methods</h3><p>This prospective case-control study was carried out with 505 pregnant women: 140 women had GDM, and 105 with PE. Remaining 260 women were age- and frequency-matched controls. TNF-α (–C850T) genotyping was determined by polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) analysis.</p></div><div><h3>Result</h3><p>We found no statistically significant difference in the genotypic and allelic distribution between GDM women and controls (for CT + TT vs. CC, χ<sup>2</sup> = 0.3919; <em>p</em> = 0.61; Odds Ratio (OR) = 0.76 (95% CI: 0.203–1.876)). No significant differences was observed in the allele and genotype frequency between PE women and controls (for CT + TT vs. CC, <em>p</em> = 0.31; OR = 0.55 (95% CI: 0.171–1.784); T vs. C, <em>p</em> = 0.71; OR = 0.94 (95% CI: 0.680–1.3)).</p></div><div><h3>Conclusion</h3><p>From our results, we conclude that the (–C850T) promoter polymorphism has no role in the propensity of pregnant women from south Indian populations to develop GDM or PE.</p></div>","PeriodicalId":91915,"journal":{"name":"Journal of reproductive health and medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jrhm.2015.01.001","citationCount":"17","resultStr":"{\"title\":\"Tumor necrosis factor alpha promoter polymorphism studies in pregnant women\",\"authors\":\"Imran Ali Khan , Vasundhara Kamineni , Subhadra Poornima , Parveen Jahan , Qurratulain Hasan , Pragna Rao\",\"doi\":\"10.1016/j.jrhm.2015.01.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Aims</h3><p>The aim of this study was to explore the possible association between the −850<!--> <span><span><span>C/T polymorphism in the tumor necrosis factor alpha (TNF-α) gene promoter, and pregnancy-associated diseases such as </span>gestational diabetes mellitus (GDM) and </span>preeclampsia<span> (PE), in south Indian women. GDM and PE are common complications that occur during pregnancy and are the leading causes of perinatal mortality. To date, the mechanisms that initiate GDM and PE in humans have remained elusive.</span></span></p></div><div><h3>Methods</h3><p>This prospective case-control study was carried out with 505 pregnant women: 140 women had GDM, and 105 with PE. Remaining 260 women were age- and frequency-matched controls. TNF-α (–C850T) genotyping was determined by polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) analysis.</p></div><div><h3>Result</h3><p>We found no statistically significant difference in the genotypic and allelic distribution between GDM women and controls (for CT + TT vs. CC, χ<sup>2</sup> = 0.3919; <em>p</em> = 0.61; Odds Ratio (OR) = 0.76 (95% CI: 0.203–1.876)). No significant differences was observed in the allele and genotype frequency between PE women and controls (for CT + TT vs. CC, <em>p</em> = 0.31; OR = 0.55 (95% CI: 0.171–1.784); T vs. C, <em>p</em> = 0.71; OR = 0.94 (95% CI: 0.680–1.3)).</p></div><div><h3>Conclusion</h3><p>From our results, we conclude that the (–C850T) promoter polymorphism has no role in the propensity of pregnant women from south Indian populations to develop GDM or PE.</p></div>\",\"PeriodicalId\":91915,\"journal\":{\"name\":\"Journal of reproductive health and medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jrhm.2015.01.001\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of reproductive health and medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214420X15000029\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of reproductive health and medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214420X15000029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
摘要
目的本研究的目的是探讨南印度妇女肿瘤坏死因子α (TNF-α)基因启动子−850 C/T多态性与妊娠相关疾病(如妊娠糖尿病(GDM)和先兆子痫(PE))之间的可能关联。GDM和PE是妊娠期间常见的并发症,也是围产期死亡的主要原因。迄今为止,在人类中引发GDM和PE的机制仍然难以捉摸。方法本前瞻性病例对照研究纳入505例孕妇,其中GDM 140例,PE 105例。其余260名女性作为年龄和频率匹配的对照组。采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)分析TNF-α (-C850T)基因分型。结果GDM女性与对照组基因型及等位基因分布差异无统计学意义(CT + TT vs. CC, χ2 = 0.3919;p = 0.61;优势比(OR) = 0.76 (95% CI: 0.203-1.876)。PE女性与对照组的等位基因和基因型频率无显著差异(CT + TT vs. CC, p = 0.31;Or = 0.55 (95% ci: 0.171-1.784);T vs. C, p = 0.71;Or = 0.94 (95% ci: 0.680-1.3))。结论(-C850T)启动子多态性与印度南部孕妇患GDM或PE的倾向无关。
Tumor necrosis factor alpha promoter polymorphism studies in pregnant women
Aims
The aim of this study was to explore the possible association between the −850 C/T polymorphism in the tumor necrosis factor alpha (TNF-α) gene promoter, and pregnancy-associated diseases such as gestational diabetes mellitus (GDM) and preeclampsia (PE), in south Indian women. GDM and PE are common complications that occur during pregnancy and are the leading causes of perinatal mortality. To date, the mechanisms that initiate GDM and PE in humans have remained elusive.
Methods
This prospective case-control study was carried out with 505 pregnant women: 140 women had GDM, and 105 with PE. Remaining 260 women were age- and frequency-matched controls. TNF-α (–C850T) genotyping was determined by polymerase chain reaction with restriction fragment length polymorphism (PCR-RFLP) analysis.
Result
We found no statistically significant difference in the genotypic and allelic distribution between GDM women and controls (for CT + TT vs. CC, χ2 = 0.3919; p = 0.61; Odds Ratio (OR) = 0.76 (95% CI: 0.203–1.876)). No significant differences was observed in the allele and genotype frequency between PE women and controls (for CT + TT vs. CC, p = 0.31; OR = 0.55 (95% CI: 0.171–1.784); T vs. C, p = 0.71; OR = 0.94 (95% CI: 0.680–1.3)).
Conclusion
From our results, we conclude that the (–C850T) promoter polymorphism has no role in the propensity of pregnant women from south Indian populations to develop GDM or PE.