Narendra Kumar, Mason Gray, Juan C. Ortiz-Marquez, Andrew Weber, Cameron R. Desmond, Avni Argun, Tim van Opijnen, Kenneth S. Burch
{"title":"用石墨烯场效应晶体管检测唾液中的多种疾病生物标志物","authors":"Narendra Kumar, Mason Gray, Juan C. Ortiz-Marquez, Andrew Weber, Cameron R. Desmond, Avni Argun, Tim van Opijnen, Kenneth S. Burch","doi":"10.1002/mds3.10121","DOIUrl":null,"url":null,"abstract":"<p>Human carbonic anhydrase 1 (CA1) has been suggested as a biomarker for identification of several diseases including cancers, pancreatitis, diabetes and Sjogren's syndrome. However, the lack of a rapid, cheap, accurate and easy-to-use quantification technique has prevented widespread utilization of CA1 for practical clinical applications. To this end, we present a label-free electronic biosensor for detection of CA1 utilizing highly sensitive graphene field effect transistors (G-FETs) as a transducer and specific RNA aptamers as a probe. The binding of CA1 with aptamers resulted in a positive shift in Dirac voltage <i>V<sub>D</sub></i> of the G-FETs, the magnitude of which depended on target concentration. These aptameric G-FET biosensors showed the binding affinity (<i>K<sub>D</sub></i>) of ~2.3 ng/ml (70 p<span>M</span>), which is four orders lower than that reported using a gel shift assay. This lower value of <i>K<sub>D</sub></i> enabled us to achieve a detection range (10 pg/ml –100 ng/ml) which is well in line with the clinically relevant range. These highly sensitive devices allowed us to further prove their clinical relevance by successfully detecting the presence of CA1 in human saliva samples. Utilization of this label-free biosensor could facilitate the early-stage identification of various diseases associated with changes in concentration of CAs.</p>","PeriodicalId":87324,"journal":{"name":"Medical devices & sensors","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2020-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/mds3.10121","citationCount":"0","resultStr":"{\"title\":\"Detection of a multi-disease biomarker in saliva with graphene field effect transistors\",\"authors\":\"Narendra Kumar, Mason Gray, Juan C. Ortiz-Marquez, Andrew Weber, Cameron R. Desmond, Avni Argun, Tim van Opijnen, Kenneth S. Burch\",\"doi\":\"10.1002/mds3.10121\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Human carbonic anhydrase 1 (CA1) has been suggested as a biomarker for identification of several diseases including cancers, pancreatitis, diabetes and Sjogren's syndrome. However, the lack of a rapid, cheap, accurate and easy-to-use quantification technique has prevented widespread utilization of CA1 for practical clinical applications. To this end, we present a label-free electronic biosensor for detection of CA1 utilizing highly sensitive graphene field effect transistors (G-FETs) as a transducer and specific RNA aptamers as a probe. The binding of CA1 with aptamers resulted in a positive shift in Dirac voltage <i>V<sub>D</sub></i> of the G-FETs, the magnitude of which depended on target concentration. These aptameric G-FET biosensors showed the binding affinity (<i>K<sub>D</sub></i>) of ~2.3 ng/ml (70 p<span>M</span>), which is four orders lower than that reported using a gel shift assay. This lower value of <i>K<sub>D</sub></i> enabled us to achieve a detection range (10 pg/ml –100 ng/ml) which is well in line with the clinically relevant range. These highly sensitive devices allowed us to further prove their clinical relevance by successfully detecting the presence of CA1 in human saliva samples. Utilization of this label-free biosensor could facilitate the early-stage identification of various diseases associated with changes in concentration of CAs.</p>\",\"PeriodicalId\":87324,\"journal\":{\"name\":\"Medical devices & sensors\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-09-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/mds3.10121\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical devices & sensors\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10121\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical devices & sensors","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/mds3.10121","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Detection of a multi-disease biomarker in saliva with graphene field effect transistors
Human carbonic anhydrase 1 (CA1) has been suggested as a biomarker for identification of several diseases including cancers, pancreatitis, diabetes and Sjogren's syndrome. However, the lack of a rapid, cheap, accurate and easy-to-use quantification technique has prevented widespread utilization of CA1 for practical clinical applications. To this end, we present a label-free electronic biosensor for detection of CA1 utilizing highly sensitive graphene field effect transistors (G-FETs) as a transducer and specific RNA aptamers as a probe. The binding of CA1 with aptamers resulted in a positive shift in Dirac voltage VD of the G-FETs, the magnitude of which depended on target concentration. These aptameric G-FET biosensors showed the binding affinity (KD) of ~2.3 ng/ml (70 pM), which is four orders lower than that reported using a gel shift assay. This lower value of KD enabled us to achieve a detection range (10 pg/ml –100 ng/ml) which is well in line with the clinically relevant range. These highly sensitive devices allowed us to further prove their clinical relevance by successfully detecting the presence of CA1 in human saliva samples. Utilization of this label-free biosensor could facilitate the early-stage identification of various diseases associated with changes in concentration of CAs.