龈沟液中的microrna:牙周炎患者差异表达的观察性病例对照研究。

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Non-Coding RNA Pub Date : 2023-11-18 DOI:10.3390/ncrna9060073

Pedro J Almiñana-Pastor, Francisco M Alpiste-Illueca, Pablo Micó-Martinez, Jose Luis García-Giménez, Eva García-López, Andrés López-Roldán

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引用次数: 0

摘要

目的:慢性牙周炎患者龈沟液(GCF)中存在的microRNAs (miRNAs)可能作为牙周病的生物标志物。本研究的目的是对GCF中存在的所有miRNA进行miRNA测序研究，比较晚期慢性牙周炎(CP)患者和健康受试者(HS)的miRNA表达水平。材料与方法:分别采集重度CP患者(n = 11)和HS患者(n = 12)单根牙GCF标本。使用miRNeasy血清/血浆试剂盒(Qiagen GmbH, Hilden, Germany)从GCF中分离mirna。采用逆转录聚合酶链反应(qRT-PCR)检测参与牙周发病的候选miRNA的表达水平。结果:在所有测序的mirna中，miR-199、miR-146a、miR-30a和miR-338被确定为最能代表CP患者样本的mirna。验证研究确定miR-199是用于定义牙周炎的最有效的生物标志物。结论:在首次对GCF中所有已知的mirna进行测序后，我们发现了几个潜在的生物标志物来定义牙周炎。利用高通量方法鉴定GCF中的miRNAS将阐明这些分子在牙周炎中的作用，并提供具有潜在应用价值的生物标志物。

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MicroRNAs in Gingival Crevicular Fluid: An Observational Case-Control Study of Differential Expression in Periodontitis.

Objectives: microRNAs (miRNAs) present in the gingival crevicular fluid (GCF) of patients with chronic periodontitis may serve as biomarkers of periodontal disease. The aim of this study was to perform a miRNA-sequencing study of all miRNAs present in GCF, comparing miRNA expression level profiles between advanced chronic periodontitis (CP) patients and healthy subjects (HS).

Materials and methods: GCF samples were collected from the single-rooted teeth of patients with severe CP (n = 11) and of HS (n = 12). miRNAs were isolated from GCF using an miRNeasy Serum/Plasma kit(Qiagen GmbH, Hilden, Germany). Reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the expression levels of miRNA candidates involved in periodontal pathogenesis.

Results: Of all the sequenced miRNAs, miR-199, miR-146a, miR-30a, and miR-338 were identified as best representing the CP patient samples. The validation study identified miR-199 as the most powerful biomarker used to define periodontitis.

Conclusions: Upon sequencing all known miRNAs in GCF for the first time, we uncovered several potential biomarkers to define periodontitis. Identifying miRNAS in the GCF using high-throughput approaches will clarify the role of these molecules in periodontitis and provide biomarkers with potential applications.

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.

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