一种与珊瑚有关的放线菌减轻了高温下珊瑚的白化。

IF 6.2 2区 环境科学与生态学 Q1 GENETICS & HEREDITY Environmental Microbiome Pub Date : 2023-11-23 DOI:10.1186/s40793-023-00540-7
Jie Li, Yiyang Zou, Qiqi Li, Jian Zhang, David G Bourne, Yuanjiao Lyu, Cong Liu, Si Zhang
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引用次数: 0

摘要

背景:已经报道了珊瑚暴露于微生物的积极影响,但人们对这些好处是如何产生的却知之甚少。在这里,我们从达米角Pocillopora damicornis中分离出一株放线菌菌株(SCSIO 13291),该菌株具有合成抗氧化剂、维生素、抗菌和抗病毒化合物的能力,并得到表型和/或基因组证据的支持。用5(和- 6)-羧基四甲基罗丹明、琥珀酰亚胺酯对菌株SCSIO 13291进行标记,并将标记的细胞悬浮液直接接种到珊瑚虫组织中,进行热应激实验。然后,我们将标记的细菌细胞可视化,并分析珊瑚的生理,转录组和微生物组,以阐明该菌株在热应激下对珊瑚全息生物的影响。结果:随后的显微镜观察证实了附着在珊瑚虫上的细菌的存在。添加SCSIO 13291菌株减少了受热应力影响的珊瑚的白化迹象。与此同时,在珊瑚宿主和共生体科种群中观察到基因表达的变化,这些变化涉及活性氧和光损伤缓解、细胞凋亡减弱和胞吐以及代谢物利用。此外,与珊瑚相关的细菌群落在接种菌株的样品中发生了更稳定的生态网络变化。结论:我们的研究结果提供了一种假定的放线菌益生菌菌株减轻珊瑚白化迹象的好处。这项研究表明,接种细菌可以通过赋予代谢活动或通过提供额外营养来源的间接机制,潜在地直接使珊瑚全息生物受益。
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A coral-associated actinobacterium mitigates coral bleaching under heat stress.

Background: The positive effects of exposing corals to microorganisms have been reported though how the benefits are conferred are poorly understood. Here, we isolated an actinobacterial strain (SCSIO 13291) from Pocillopora damicornis with capabilities to synthesize antioxidants, vitamins, and antibacterial and antiviral compounds supported with phenotypic and/or genomic evidence. Strain SCSIO 13291 was labeled with 5 (and - 6)-carboxytetramethylrhodamine, succinimidyl ester and the labeled cell suspension directly inoculated onto the coral polyp tissues when nubbins were under thermal stress in a mesocosm experiment. We then visualized the labelled bacterial cells and analyzed the coral physiological, transcriptome and microbiome to elucidate the effect this strain conferred on the coral holobiont under thermal stress.

Results: Subsequent microscopic observations confirmed the presence of the bacterium attached to the coral polyps. Addition of the SCSIO 13291 strain reduced signs of bleaching in the corals subjected to heat stress. At the same time, alterations in gene expression, which were involved in reactive oxygen species and light damage mitigation, attenuated apoptosis and exocytosis in addition to metabolite utilization, were observed in the coral host and Symbiodiniaceae populations. In addition, the coral associated bacterial community altered with a more stable ecological network for samples inoculated with the bacterial strain.

Conclusions: Our results provide insights into the benefits of a putative actinobacterial probiotic strain that mitigate coral bleaching signs. This study suggests that the inoculation of bacteria can potentially directly benefit the coral holobiont through conferring metabolic activities or through indirect mechanisms of suppling additional nutrient sources.

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来源期刊
Environmental Microbiome
Environmental Microbiome Immunology and Microbiology-Microbiology
CiteScore
7.40
自引率
2.50%
发文量
55
审稿时长
13 weeks
期刊介绍: Microorganisms, omnipresent across Earth's diverse environments, play a crucial role in adapting to external changes, influencing Earth's systems and cycles, and contributing significantly to agricultural practices. Through applied microbiology, they offer solutions to various everyday needs. Environmental Microbiome recognizes the universal presence and significance of microorganisms, inviting submissions that explore the diverse facets of environmental and applied microbiological research.
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