{"title":"DNA甲基化年龄估计模型在中国东部汉族人群中的跨平台应用","authors":"Zi-Wei Wang, Cheng-Tao Li, Xi-Ling Liu","doi":"10.12116/j.issn.1004-5619.2022.520405","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.</p><p><strong>Methods: </strong>According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.</p><p><strong>Results: </strong>The 6 DNA methylation sites in both detection techniques were age-related, with an <i>R</i><sup>2</sup> of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an <i>R</i><sup>2</sup> of 0.84 and MAD of 4.41 years when using NGS.</p><p><strong>Conclusions: </strong>The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.</p>","PeriodicalId":15899,"journal":{"name":"Journal of Forensic Medicine","volume":"39 5","pages":"441-446"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population.\",\"authors\":\"Zi-Wei Wang, Cheng-Tao Li, Xi-Ling Liu\",\"doi\":\"10.12116/j.issn.1004-5619.2022.520405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.</p><p><strong>Methods: </strong>According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.</p><p><strong>Results: </strong>The 6 DNA methylation sites in both detection techniques were age-related, with an <i>R</i><sup>2</sup> of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an <i>R</i><sup>2</sup> of 0.84 and MAD of 4.41 years when using NGS.</p><p><strong>Conclusions: </strong>The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.</p>\",\"PeriodicalId\":15899,\"journal\":{\"name\":\"Journal of Forensic Medicine\",\"volume\":\"39 5\",\"pages\":\"441-446\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Forensic Medicine\",\"FirstCategoryId\":\"90\",\"ListUrlMain\":\"https://doi.org/10.12116/j.issn.1004-5619.2022.520405\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Forensic Medicine","FirstCategoryId":"90","ListUrlMain":"https://doi.org/10.12116/j.issn.1004-5619.2022.520405","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population.
Objectives: To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.
Methods: According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.
Results: The 6 DNA methylation sites in both detection techniques were age-related, with an R2 of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an R2 of 0.84 and MAD of 4.41 years when using NGS.
Conclusions: The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.
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