Sanja Zornic, Bojana Lukovic, Ivana Petrovic, Aleksandra Jencic
{"title":"血液培养中多重耐药革兰氏阴性菌的流行及多重PCR快速检测β -内酰胺酶编码基因。","authors":"Sanja Zornic, Bojana Lukovic, Ivana Petrovic, Aleksandra Jencic","doi":"10.18683/germs.2022.1349","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to determine the prevalence of multidrug-resistant Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and the multiplex PCR assay's ability to detect resistance genes.</p><p><strong>Methods: </strong>A total of 388 GNB isolates obtained from hospitalized patients between November 2019 and November 2021 were included in the study. Antimicrobial susceptibility testing was done by VITEK 2 system and broth microdilution method. Beta-lactamase-encoding genes were detected by multiplex PCR assays, BioFire-Blood Culture Identification 2 (BCID2) panel (bioMérieux, France). Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK AST-GN71 card (bioMérieux, France). The isolates of GNB were classified into multidrug-resistant, extensively-drug-resistant, and pandrug-resistant categories, and their prevalence and distribution in different wards, including coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated.</p><p><strong>Results: </strong>Results revealed that all isolates of <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> were multidrug-resistant as well as 91.6% of <i>Enterobacter cloacae</i>, 80.6% of <i>Proteus mirabilis</i>, and 76.1% of <i>Klebsiella pneumoniae</i>, respectively. In fermentative bacteria, <i>bla</i><sub>OXA-48-like</sub> (58.1%), <i>bla</i><sub>NDM</sub> (16.1%), <i>bla</i><sub>KPC</sub> (9.7%) and <i>bla</i><sub>VIM</sub> (6.5%) genes were detected. More than half of <i>Enterobacter cloacae</i> (58.3%) and <i>Klebsiella pneumoniae</i> (53.7%) produced ESBLs. Among non-fermenters, the <i>bla</i><sub>NDM</sub> gene was carried by 55% of <i>Pseudomonas aeruginosa</i> and 19.5% of <i>Acinetobacter baumannii</i>. In the COVID-19 ICU, <i>Acinetobacter baumannii</i> was the most common isolate (86.1%).</p><p><strong>Conclusions: </strong>This study revealed high proportions of multidrug-resistant blood isolates and various underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be helpful for the detection of the most prevalent resistance genes of fermentative bacteria.</p>","PeriodicalId":45107,"journal":{"name":"GERMS","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660225/pdf/","citationCount":"0","resultStr":"{\"title\":\"Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and rapid detection of beta-lactamase-encoding genes by multiplex PCR assay.\",\"authors\":\"Sanja Zornic, Bojana Lukovic, Ivana Petrovic, Aleksandra Jencic\",\"doi\":\"10.18683/germs.2022.1349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>This study aimed to determine the prevalence of multidrug-resistant Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and the multiplex PCR assay's ability to detect resistance genes.</p><p><strong>Methods: </strong>A total of 388 GNB isolates obtained from hospitalized patients between November 2019 and November 2021 were included in the study. Antimicrobial susceptibility testing was done by VITEK 2 system and broth microdilution method. Beta-lactamase-encoding genes were detected by multiplex PCR assays, BioFire-Blood Culture Identification 2 (BCID2) panel (bioMérieux, France). Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK AST-GN71 card (bioMérieux, France). The isolates of GNB were classified into multidrug-resistant, extensively-drug-resistant, and pandrug-resistant categories, and their prevalence and distribution in different wards, including coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated.</p><p><strong>Results: </strong>Results revealed that all isolates of <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> were multidrug-resistant as well as 91.6% of <i>Enterobacter cloacae</i>, 80.6% of <i>Proteus mirabilis</i>, and 76.1% of <i>Klebsiella pneumoniae</i>, respectively. In fermentative bacteria, <i>bla</i><sub>OXA-48-like</sub> (58.1%), <i>bla</i><sub>NDM</sub> (16.1%), <i>bla</i><sub>KPC</sub> (9.7%) and <i>bla</i><sub>VIM</sub> (6.5%) genes were detected. More than half of <i>Enterobacter cloacae</i> (58.3%) and <i>Klebsiella pneumoniae</i> (53.7%) produced ESBLs. Among non-fermenters, the <i>bla</i><sub>NDM</sub> gene was carried by 55% of <i>Pseudomonas aeruginosa</i> and 19.5% of <i>Acinetobacter baumannii</i>. In the COVID-19 ICU, <i>Acinetobacter baumannii</i> was the most common isolate (86.1%).</p><p><strong>Conclusions: </strong>This study revealed high proportions of multidrug-resistant blood isolates and various underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be helpful for the detection of the most prevalent resistance genes of fermentative bacteria.</p>\",\"PeriodicalId\":45107,\"journal\":{\"name\":\"GERMS\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2022-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10660225/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"GERMS\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18683/germs.2022.1349\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/12/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"GERMS","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18683/germs.2022.1349","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/12/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Prevalence of multidrug-resistant Gram-negative bacteria from blood cultures and rapid detection of beta-lactamase-encoding genes by multiplex PCR assay.
Introduction: This study aimed to determine the prevalence of multidrug-resistant Gram-negative bacteria (GNB) from blood cultures in a tertiary-care hospital and the multiplex PCR assay's ability to detect resistance genes.
Methods: A total of 388 GNB isolates obtained from hospitalized patients between November 2019 and November 2021 were included in the study. Antimicrobial susceptibility testing was done by VITEK 2 system and broth microdilution method. Beta-lactamase-encoding genes were detected by multiplex PCR assays, BioFire-Blood Culture Identification 2 (BCID2) panel (bioMérieux, France). Extended-spectrum beta-lactamases (ESBLs) were detected phenotypically with VITEK AST-GN71 card (bioMérieux, France). The isolates of GNB were classified into multidrug-resistant, extensively-drug-resistant, and pandrug-resistant categories, and their prevalence and distribution in different wards, including coronavirus diseases 2019 (COVID-19) intensive care units (ICU), were calculated.
Results: Results revealed that all isolates of Acinetobacter baumannii and Pseudomonas aeruginosa were multidrug-resistant as well as 91.6% of Enterobacter cloacae, 80.6% of Proteus mirabilis, and 76.1% of Klebsiella pneumoniae, respectively. In fermentative bacteria, blaOXA-48-like (58.1%), blaNDM (16.1%), blaKPC (9.7%) and blaVIM (6.5%) genes were detected. More than half of Enterobacter cloacae (58.3%) and Klebsiella pneumoniae (53.7%) produced ESBLs. Among non-fermenters, the blaNDM gene was carried by 55% of Pseudomonas aeruginosa and 19.5% of Acinetobacter baumannii. In the COVID-19 ICU, Acinetobacter baumannii was the most common isolate (86.1%).
Conclusions: This study revealed high proportions of multidrug-resistant blood isolates and various underlying resistance genes in Gram-negative strains. The BCID2 panel seems to be helpful for the detection of the most prevalent resistance genes of fermentative bacteria.
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