木麻黄皮金纳米提取物对毒死蜱致大鼠肝神经保护作用的研究。

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-12-01 DOI:10.1186/s43141-023-00595-6
Wael Mahmoud Aboulthana, Noha El-Sayed Ibrahim, Amgad Kamal Hassan, Wagdy Khalil Bassaly, Hassan Abdel-Gawad, Hamdy Ahmed Taha, Kawkab A Ahmed
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摘要

背景:木麻黄树皮含有多种活性植物成分,适合于生物合成金纳米颗粒(Au-NPs)。这些纳米颗粒随后被评估其降低毒死蜱(CPF)对大鼠的毒性的有效性。结果:本研究进行了各种血液学和生化测量。此外,对肝脏和脑组织中氧化应激和炎症反应的标志物进行量化评估。肝、脑组织均行组织病理学检查。此外,我们还分析了天然电泳蛋白和同工酶图谱,并测定了凋亡基因在这些组织中的相对表达水平。注射CPF组血液学和生化指标发生了严重改变。然而,Au-C的管理。在所有处理组中,木贼叶纳米提取物使这些水平正常化。cpf注射组抗氧化系统标志物显著降低(P≤0.05),肝脏和脑组织炎症和纤维化标志物水平升高。相比之下,与cpf注射组相比,预处理组在使用纳米提取物处理时表现出这些标记物的减少。此外,纳米提取物减轻了CPF在肝脏和脑组织中引起的组织病理学病变的严重程度,且预处理组的改善效果更高。对肝脏和脑组织进行的电泳分析显示,纳米提取物阻止了预处理组CPF引起的质变。此外,分子分析显示cpf注射大鼠中凋亡基因的相对表达显著增加。虽然与cpf注射组相比,纳米提取物改善了这些基因的相对表达,但无法将其值恢复到正常水平。结论:纳米提取物在血液学、生化、组织病理学、生理学和分子水平上均能有效降低CPF对大鼠的毒性。
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The hepato- and neuroprotective effect of gold Casuarina equisetifolia bark nano-extract against Chlorpyrifos-induced toxicity in rats.

Background: The bark of Casuarina equisetifolia contains several active phytoconstituents that are suitable for the biosynthesis of gold nanoparticles (Au-NPs). These nanoparticles were subsequently evaluated for their effectiveness in reducing the toxicity induced by Chlorpyrifos (CPF) in rats.

Results: Various hematological and biochemical measurements were conducted in this study. In addition, markers of oxidative stress and inflammatory reactions quantified in liver and brain tissues were evaluated. Histopathological examinations were performed on both liver and brain tissues. Furthermore, the native electrophoretic protein and isoenzyme patterns were analyzed, and the relative expression levels of apoptotic genes in these tissues were determined. The hematological and biochemical parameters were found to be severely altered in the group injected with CPF. However, the administration of Au-C. equisetifolia nano-extract normalized these levels in all treated groups. The antioxidant system markers showed a significant decrease (P ≤ 0.05) in conjunction with elevated levels of inflammatory and fibrotic markers in both liver and brain tissues of the CPF-injected group. In comparison, the pre-treated group exhibited a reduction in these markers when treated with the nano-extract, as opposed to the CPF-injected group. Additionally, the nano-extract mitigated the severity of histopathological lesions induced by CPF in both liver and brain tissues, with a higher ameliorative effect observed in the pre-treated group. Electrophoretic assays conducted on liver and brain tissues revealed that the nano-extract prevented the qualitative changes induced by CPF in the pre-treated group. Furthermore, the molecular assay demonstrated a significant increase in the relative expression of apoptotic genes in the CPF-injected rats. Although the nano-extract ameliorated the relative expression of these genes compared to the CPF-injected group, it was unable to restore their values to normal levels.

Conclusion: Our results demonstrated that the nano-extract effectively reduced the toxicity induced by CPF in rats at hematological, biochemical, histopathological, physiological, and molecular levels, in the group pre-treated with the nano-extract.

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