探索鼻咽癌中 SNHG1 的表达及其对 PI3K-AKT 轴的影响。

IF 2 4区 医学 Q3 ONCOLOGY Neoplasma Pub Date : 2023-10-01 DOI:10.4149/neo_2023_230517N263
Yong Yang, Yan-Ping Yang, Mei-Ling Yi, Fang-Ting Huang, Xia Zhu, Guang-Wu Huang
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引用次数: 0

摘要

放疗和化疗提高了鼻咽癌患者的五年生存率,但副作用普遍导致临床疗效不理想。当务之急是探索鼻咽癌的发病机制,以找到更好的诊断和治疗方法。小核仁RNA宿主基因(SNHGs)是一种特殊的lncRNA,可进一步剪接产生小核仁RNA(snoRNAs)。SNHG1 已被发现与多种癌症相关。然而,只有少数研究报告了 SNHG1 与鼻咽癌之间的关系。本研究首先通过生物信息学分析了 SNHG1 在鼻咽癌中的诊断性能和相关信号通路。通过 RT-qPCR 验证了 SNHG1 的表达,并通过免疫组化检测了信号通路的表达。生物信息学分析结果显示,SNHG1在头颈部鳞状细胞癌(HNSC)和鼻咽癌组织中明显过表达。RT-qPCR 检测证实 SNHG1 在鼻咽癌组织中明显过表达。富集分析表明,SNHG1可能通过PI3K-AKT信号通路作用于鼻咽癌。免疫组化实验显示,在鼻咽癌组织中,PI3K-AKT 信号通路蛋白(PI3K AKT 和 EGFR)呈阳性表达,而 CASP3 呈弱阳性表达。因此,我们认为 SNHG1 是一种前瞻性生物标记物,可能通过 PI3K-AKT 信号通路作用于鼻咽癌。
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Exploring the expression of SNHG1 and its effect on the PI3K-AKT axis in nasopharyngeal cancer.

Radiotherapy and chemotherapy have improved the 5-year survival rate of nasopharyngeal carcinoma (NPC) patients, but the side effects generally lead to unsatisfactory clinical efficacy. It's imperative to explore the pathogenesis of NPC to find better diagnostic and therapeutic methods. Small nucleolar RNA host genes (SNHGs) are special lncRNAs, which can be further spliced to produce small nucleolar RNAs (snoRNAs). SNHG1 has been found to be associated with various cancers. However, only a few studies reported the relationship between SNHG1 and NPC. This study first analyzed the diagnostic performance and related signaling pathways of SNHG1 in NPC through bioinformatics. The expression of SNHG1 was verified by RT-qPCR, and the expression of the signaling pathway was detected using immunohistochemistry. Bioinformatics analysis results showed that SNHG1 was significantly overexpressed in head and neck squamous cell carcinoma (HNSC) and NPC tissues. RT-qPCR detection confirmed the significant overexpression of SNHG1 in NPC tissues. Enrichment analysis showed that SNHG1 may act on NPC through the PI3K-AKT signaling pathway. Immunohistochemistry experiment revealed PI3K-AKT signaling pathway proteins (PI3K AKT and EGFR) positively expressed and CASP3 weakly positively expressed in NPC tissues. Therefore, we concluded that SNHG1 is a prospective biomarker and may act on NPC through the PI3K-AKT signaling pathway.

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来源期刊
Neoplasma
Neoplasma 医学-肿瘤学
CiteScore
5.40
自引率
0.00%
发文量
238
审稿时长
3 months
期刊介绍: The journal Neoplasma publishes articles on experimental and clinical oncology and cancer epidemiology.
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