Kisspeptin在体外通过同源受体刺激绵羊卵巢卵泡发育。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-01 Epub Date: 2023-12-07 DOI:10.1017/S096719942300059X
B Divya Sri, S Harsha Lekha, K Narendra Gopal Reddy, Deepa Pathipati, B Rambabu Naik, P Jagapathy Ramayya, K Veera Bramhaiah, L S S Varaprasad Reddy, A V N Siva Kumar
{"title":"Kisspeptin在体外通过同源受体刺激绵羊卵巢卵泡发育。","authors":"B Divya Sri, S Harsha Lekha, K Narendra Gopal Reddy, Deepa Pathipati, B Rambabu Naik, P Jagapathy Ramayya, K Veera Bramhaiah, L S S Varaprasad Reddy, A V N Siva Kumar","doi":"10.1017/S096719942300059X","DOIUrl":null,"url":null,"abstract":"<p><p>The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the <i>in vitro</i> development of preantral follicles (PFs) and (2) evolution of KP receptor gene (<i>KISS1R</i>) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0-100 µg/ml) in the culture medium of PFs for 6 days. The cumulus-oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development <i>in vitro</i>. The expression of the kisspeptin receptor gene at each development stages of <i>in vivo</i> developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and <i>in vitro</i> cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The <i>KISS1R</i> gene was expressed in follicular cells and oocytes at all the development stages of both <i>in vivo</i> developed and <i>in vitro</i> cultured follicles. Higher <i>KISS1R</i> gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Kisspeptin stimulates sheep ovarian follicular development <i>in vitro</i> through homologous receptors.\",\"authors\":\"B Divya Sri, S Harsha Lekha, K Narendra Gopal Reddy, Deepa Pathipati, B Rambabu Naik, P Jagapathy Ramayya, K Veera Bramhaiah, L S S Varaprasad Reddy, A V N Siva Kumar\",\"doi\":\"10.1017/S096719942300059X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the <i>in vitro</i> development of preantral follicles (PFs) and (2) evolution of KP receptor gene (<i>KISS1R</i>) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0-100 µg/ml) in the culture medium of PFs for 6 days. The cumulus-oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development <i>in vitro</i>. The expression of the kisspeptin receptor gene at each development stages of <i>in vivo</i> developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and <i>in vitro</i> cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The <i>KISS1R</i> gene was expressed in follicular cells and oocytes at all the development stages of both <i>in vivo</i> developed and <i>in vitro</i> cultured follicles. Higher <i>KISS1R</i> gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1017/S096719942300059X\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1017/S096719942300059X","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

摘要

本研究旨在阐明(1)kisspeptin (KP)对绵羊体外腔前卵泡(PFs)发育的影响和(2)KP受体基因(KISS1R)在卵巢卵泡发育过程中的表达进化。在PFs培养液中添加Kisspeptin(0-100µg/ml) 6天。培养的PFs的卵丘-卵母细胞复合物(COCs)随后再成熟24小时至中期II期(MII)。显示生长、腔形成、平均直径增加和卵母细胞成熟到MII期的PFs比例是体外卵泡发育的指标。利用实时聚合酶链反应(real-time polymerase chain reaction)评估kisspeptin受体基因在体内发育的各个发育阶段(胃窦前、早期、胃窦、大胃窦和来自Graafian卵泡的COCs)和体外培养的补充了KP的PFs的表达。当KP浓度为10µg/ml时,各参数发育最佳。在含有其他生长因子(胰岛素样生长因子- 1)和激素(生长激素、甲状腺素、促卵泡激素)的培养基中补充KP(10µg/ml)可促进PF的发育。KISS1R基因在体内发育和体外培养卵泡的所有发育阶段的卵泡细胞和卵母细胞中均有表达。在含有KP和其他激素和生长因子的培养基中支持KISS1R基因的高表达。因此,我们认为KP等生长因子和激素影响哺乳动物卵巢卵泡发育的机制之一是通过上调KP受体基因的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Kisspeptin stimulates sheep ovarian follicular development in vitro through homologous receptors.

The present study was conducted to elucidate (1) the influence of kisspeptin (KP) on the in vitro development of preantral follicles (PFs) and (2) evolution of KP receptor gene (KISS1R) expression during ovarian follicular development in sheep. Kisspeptin was supplemented (0-100 µg/ml) in the culture medium of PFs for 6 days. The cumulus-oocyte complexes (COCs) from cultured PFs were subsequently matured to metaphase II (MII) for an additional 24 h. The proportions of PFs exhibiting growth, antrum formation, average increase in diameter, and maturation of oocytes to MII stage were the indicators of follicular development in vitro. The expression of the kisspeptin receptor gene at each development stages of in vivo developed (preantral, early antral, antral, large antral and COCs from Graafian follicles) and in vitro cultured PFs supplemented with KP was assessed using a real-time polymerase chain reaction. The best development in all the parameters under study was elicited with 10 µg/ml of KP. Supplementation of KP (10 µg/ml) in a medium containing other growth factors (insulin-like growth factor-I) and hormones (growth hormone, thyroxine, follicle-stimulating hormone) resulted in better PF development. The KISS1R gene was expressed in follicular cells and oocytes at all the development stages of both in vivo developed and in vitro cultured follicles. Higher KISS1R gene expression was supported by culture medium containing KP along with other hormones and growth factors. Accordingly, it is suggested that one of the mechanisms through which KP and other growth factors and hormones influence the ovarian follicular development in mammals is through the upregulation of expression of the KP receptor gene.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
期刊最新文献
Management of Cholesteatoma: Hearing Rehabilitation. Congenital Cholesteatoma. Evaluation of Cholesteatoma. Management of Cholesteatoma: Extension Beyond Middle Ear/Mastoid. Recidivism and Recurrence.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1