膜胆固醇富集和叶酸功能化导致小鼠卵巢腹腔内肿瘤植入物中红细胞来源的光学纳米构建物的积累增加

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2023-12-05 DOI:10.1016/j.nano.2023.102728
Chi-Hua Lee PhD , Jenny Mac PhD , Taylor Hanley PhD , Shamima Zaman MS , Raviraj Vankayala PhD , Bahman Anvari PhD
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摘要

细胞减少手术仍然是治疗卵巢癌的金标准,但由于并非所有肿瘤都能术中可见切除,其疗效有限。我们设计了红细胞衍生的纳米结构,封装近红外(NIR)荧光团,吲哚菁绿(ICG),作为近红外荧光成像卵巢肿瘤的光学探针。在这里,我们用胆固醇丰富了这些纳米结构的膜,并在其表面用叶酸(FA)功能化,以靶向叶酸受体-α。通过小鼠模型,我们发现,在注射后24小时,具有膜胆固醇富集和FA功能化的纳米构建物的每个肿瘤块的平均注射剂量比未包封的ICG高6倍,比仅富含胆固醇的纳米构建物高2倍,比仅具有FA功能化的纳米构建物高33. %。这些结果表明,含有胆固醇和FA的红细胞衍生纳米结构为改善卵巢肿瘤的荧光成像提供了一个平台。
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Membrane cholesterol enrichment and folic acid functionalization lead to increased accumulation of erythrocyte-derived optical nano-constructs within the ovarian intraperitoneal tumor implants in mice

Cytoreductive surgery remains as the gold standard to treat ovarian cancer, but with limited efficacy since not all tumors can be intraoperatively visualized for resection. We have engineered erythrocyte-derived nano-constructs that encapsulate the near infrared (NIR) fluorophore, indocyanine green (ICG), as optical probes for NIR fluorescence imaging of ovarian tumors. Herein, we have enriched the membrane of these nano-constructs with cholesterol, and functionalized their surface with folic acid (FA) to target the folate receptor-α. Using a mouse model, we show that the average fraction of the injected dose per tumor mass for nano-constructs with both membrane cholesterol enrichment and FA functionalization was ~ sixfold higher than non-encapsulated ICG, ~ twofold higher than nano-constructs enriched with cholesterol alone, and 33 % higher than nano-constructs with only FA functionalization at 24-h post-injection. These results suggest that erythrocyte-derived nano-constructs containing both cholesterol and FA present a platform for improved fluorescence imaging of ovarian tumors.

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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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