通过等离子体增强干扰无标记跟踪蛋白质

IF 4.8 Q2 NANOSCIENCE & NANOTECHNOLOGY ACS Nanoscience Au Pub Date : 2023-11-19 DOI:10.1021/acsnanoscienceau.3c00045
Matthew Peters, Declan McIntosh, Alexandra Branzan Albu, Cuifeng Ying and Reuven Gordon*, 
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引用次数: 0

摘要

通过干涉成像方法可以观察和表征溶液中单个未修饰的生物分子;然而,瑞利散射将其限制在较大的蛋白质(通常为30 kDa)。利用金属薄膜孔径发射表面等离子体增强的干涉成像技术,我们观察了低至14 kDa的未修饰蛋白质的实时图像跟踪。较大的蛋白质表现出较慢的扩散,通过跟踪来量化。当扩散的蛋白质最终被纳米孔捕获时,我们进行互补功率谱密度和噪声幅度分析,从而获得蛋白质的信息。这种方法允许用最少的样品制备快速蛋白质表征,并打开了实时表征蛋白质相互作用的大门。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Label-Free Tracking of Proteins through Plasmon-Enhanced Interference

Single unmodified biomolecules in solution can be observed and characterized by interferometric imaging approaches; however, Rayleigh scattering limits this to larger proteins (typically >30 kDa). We observe real-time image tracking of unmodified proteins down to 14 kDa using interference imaging enhanced by surface plasmons launched at an aperture in a metal film. The larger proteins show slower diffusion, quantified by tracking. When the diffusing protein is finally trapped by the nanoaperture, we perform complementary power spectral density and noise amplitude analysis, which gives information about the protein. This approach allows for rapid protein characterization with minimal sample preparation and opens the door to characterizing protein interactions in real time.

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来源期刊
ACS Nanoscience Au
ACS Nanoscience Au 材料科学、纳米科学-
CiteScore
4.20
自引率
0.00%
发文量
0
期刊介绍: ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
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