高速原子力显微镜拍摄蛋白质分子的动态动作

IF 10.4 1区生物学 Q1 BIOPHYSICS Annual Review of Biophysics Pub Date : 2023-12-07 DOI:10.1146/annurev-biophys-030722-113353

Toshio Ando, Shingo Fukuda, Kien X. Ngo, Holger Flechsig

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引用次数: 0

摘要

结构生物学目前正在向动态结构生物学转变，动态结构生物学揭示了蛋白质在功能活动过程中的动态结构，从而更好地阐明了蛋白质的功能。在动态结构生物学的各种方法中，高速原子力显微镜（HS-AFM）是独一无二的，它能够拍摄单个分子的动态活动，但只能获得地形信息。本综述为如何使用 HS-AFM 进行生物分子成像提供了指导，并展示了几个实例，同时还介绍了 HS-AFM 技术的最新进展。最后，我们讨论了 HS-AFM 在即将到来的动态结构生物学时代的前景。《生物物理学年刊》（Annual Review of Biophysics）第 53 卷的最终在线出版日期预计为 2024 年 5 月。修订后的预计日期请参见 http://www.annualreviews.org/page/journal/pubdates。

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High-Speed Atomic Force Microscopy for Filming Protein Molecules in Dynamic Action

Structural biology is currently undergoing a transformation into dynamic structural biology, which reveals the dynamic structure of proteins during their functional activity to better elucidate how they function. Among the various approaches in dynamic structural biology, high-speed atomic force microscopy (HS-AFM) is unique in the ability to film individual molecules in dynamic action, although only topographical information is acquirable. This review provides a guide to the use of HS-AFM for biomolecular imaging and showcases several examples, as well as providing information on up-to-date progress in HS-AFM technology. Finally, we discuss the future prospects of HS-AFM in the context of dynamic structural biology in the upcoming era.Expected final online publication date for the Annual Review of Biophysics, Volume 53 is May 2024. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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来源期刊

Annual Review of Biophysics 生物-生物物理

CiteScore

21.00

自引率

0.00%

发文量

期刊介绍： The Annual Review of Biophysics, in publication since 1972, covers significant developments in the field of biophysics, including macromolecular structure, function and dynamics, theoretical and computational biophysics, molecular biophysics of the cell, physical systems biology, membrane biophysics, biotechnology, nanotechnology, and emerging techniques.