从甘薯果肉中分离出的丝状真菌的淀粉酶潜力

Vanessa Kamila Mello Grizafis, Jaime Alberti Gomes, Sônia Alvim Veiga Pileggi, Sandra Regina Masetto Antunes, Nátali Maidl de Souza, Pedro Henrique Weirich Neto
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引用次数: 0

摘要

甘薯是一种乡村文化,广泛适应,淀粉含量高,因此有无数的天赋。将红薯转化为生物燃料,需要经过一些过程。红薯生产乙醇的成功取决于淀粉转化为可发酵糖。最常用的转化过程是酶水解,它使用市售的酶。然而,这个过程可以通过丝状真菌分泌的酶来完成。在这项工作中,研究了自然定植红薯的丝状真菌。将它们分离出来,进行酶活性测试,并通过微培养进行鉴定。从甘薯Beauregard和brs - amsamulia果肉中分离到51株真菌,其中27株具有水解淀粉的能力。其中6株酶指数≥2.0,鉴定为曲霉属和青霉属。这一事实表明,这些真菌具有生产淀粉酶的潜力,而淀粉酶是生产乙醇的重要酶。
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Amylase potential of filamentous fungi isolated from sweet potato pulp
Sweet potato is a rustic culture, widely adapted and with a high starch content, thus having innumerable aptitudes. For the transformation of sweet potatoes into biofuel, some processes are necessary. The success of ethanol production from sweet potatoes depends on the transformation of starch into fermentable sugars. The most used conversion process is enzymatic hydrolysis, which uses commercially available enzymes. However, this process can be carried out through enzymes secreted by filamentous fungi. In this work, filamentous fungi that naturally colonized sweet potatoes were studied. These were isolated, tested for enzymatic activity, and identified by microculture. Fifty-one fungi from the pulp of sweet potatoes Beauregard and BRS-Amélia were isolated, 27 of them showed the ability to hydrolyze starch. Out of these, six showed an enzyme index ≥ 2.0 and were identified as Aspergillus sp. and Penicillium sp. This fact indicates the potential of these fungi in the production of amylase, an important enzyme for ethanol production.
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