{"title":"硅橡胶/聚乙烯醇/氧化锆化合物对间质基质细胞的影响","authors":"Muhammad Reza Arifianto","doi":"10.47307/gmc.2023.131.4.8","DOIUrl":null,"url":null,"abstract":"Objective: This study aimed to conduct a cytotoxicity test in determining the viability and proliferation profile for novel material of silicone rubber/polyvinyl alcohol (PVA) with or without Zirconium oxide on mesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2×105 seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statistical analysis was conducted using ANOVA, or the Kruskal-Walli’s test with a CI of 95%.","PeriodicalId":12577,"journal":{"name":"Gaceta Médica de Caracas","volume":"35 27","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Silicone Rubber/Polyvinyl Alcohol /Zirconium Oxide Compound on Mesenchymal Stromal Cells\",\"authors\":\"Muhammad Reza Arifianto\",\"doi\":\"10.47307/gmc.2023.131.4.8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: This study aimed to conduct a cytotoxicity test in determining the viability and proliferation profile for novel material of silicone rubber/polyvinyl alcohol (PVA) with or without Zirconium oxide on mesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2×105 seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statistical analysis was conducted using ANOVA, or the Kruskal-Walli’s test with a CI of 95%.\",\"PeriodicalId\":12577,\"journal\":{\"name\":\"Gaceta Médica de Caracas\",\"volume\":\"35 27\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gaceta Médica de Caracas\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.47307/gmc.2023.131.4.8\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gaceta Médica de Caracas","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47307/gmc.2023.131.4.8","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of Silicone Rubber/Polyvinyl Alcohol /Zirconium Oxide Compound on Mesenchymal Stromal Cells
Objective: This study aimed to conduct a cytotoxicity test in determining the viability and proliferation profile for novel material of silicone rubber/polyvinyl alcohol (PVA) with or without Zirconium oxide on mesenchymal stromal cell culture. Methods: An in vitro study was carried out on adipose-derived mesenchymal stromal cell culture. Samples were divided into five groups: control, silicone rubber/polyvinyl alcohol (PVA) without Zirconium oxide, silicone rubber/polyvinyl alcohol (PVA) with Zirconium oxide 1 %, 3 %, and 5 %. Each group contained 2×105 seeded MSCs/well stained with MTT for its viability. For proliferation, MTT staining was performed on days 1, 3, and 5 to assess the trend of the percentage of the living cell. Statistical analysis was conducted using ANOVA, or the Kruskal-Walli’s test with a CI of 95%.
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