P. V. Iliasov, L. V. Limareva, A. I. Sizova, V. A. Zalomlenkov, A. P. Kuricyna
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Assessment of the Toxic Effect of 2-(Chlorodinitromethyl)-4-Methoxy-6-(4-Methylpiperazine-1-yl)-1,3,5-Triazine by Respiratory Activity of Lymphocytes
A method for evaluation of metabolic characteristics of intact cells based on electrochemical registration of their respiratory activity was used to monitor a reaction of lymphocytes to a potential pharmacological agent, 2-(chlorodinitromethyl)-4-methoxy-6-(4-methylpiperazin-1-yl)-1,3,5-triazine. The method ensured an estimation of cytotoxicity of the test compound and made it possible to determine its minimum toxic concentrations for human lymphocytes. It was shown that the obtained results agree with the data of the reference method, MTT-based cell viability assay.
期刊介绍：
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.
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