短喙针鼹(Tachyglossus Aculeatus)的肠道微生物群在整个孕期表现出稳定性

IF 3.9 3区 生物学 Q2 MICROBIOLOGY MicrobiologyOpen Pub Date : 2023-12-14 DOI:10.1002/mbo3.1392
Isini Buthgamuwa, Jane C. Fenelon, Alice Roser, Haley Meer, Stephen D. Johnston, Ashley M. Dungan
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引用次数: 0

摘要

原生肠道微生物群落(微生物群)在健康中起着关键作用,可能对怀孕期间的母亲和胎儿尤其重要。单目目动物,如短喙针鼹,已经进化到可以产卵和孵化,卵孵化在幼崽觅食的育儿袋里。由于粪便和卵子都通过泄殖腔,雌性针鼹的粪便微生物群提供了将微生物垂直传播给后代的机会。在这里,我们描述了雌性短喙针鼹的肠道/粪便微生物组,并更好地了解了它们在怀孕期间可能发生的微生物组变化。从昆士兰Currumbin野生动物保护区获得4只雌性针鼹和5只雄性针鼹的粪便样本,并对其进行测序以评估细菌群落结构。我们鉴定出25种核心细菌,其中大部分存在于男性和女性样本中。Fusobacterium、Bacteroides、Escherichia-Shigella、Lactobacillus等属在男性和女性样本中均持续丰富,分别占reads的58.00%和56.14%,与性别和妊娠期无关。针鼹的微生物组成在不同的妊娠阶段保持稳定,但雌雄针鼹的微生物组成存在显著差异。这项研究首次描述了短喙针鼹在生殖阶段的微生物组组成,并允许将这种新信息用作健康指标,以帮助检测由微生物群失调引发的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Gut microbiota in the short-beaked echidna (Tachyglossus Aculeatus) shows stability across gestation

Indigenous gut microbial communities (microbiota) play critical roles in health and may be especially important for the mother and fetus during pregnancy. Monotremes, such as the short-beaked echidna, have evolved to lay and incubate an egg, which hatches in their pouch where the young feeds. Since both feces and eggs pass through the cloaca, the fecal microbiota of female echidnas provides an opportunity for vertical transmission of microbes to their offspring. Here, we characterize the gut/fecal microbiome of female short-beaked echidnas and gain a better understanding of the changes that may occur in their microbiome as they go through pregnancy. Fecal samples from four female and five male echidnas were obtained from the Currumbin Wildlife Sanctuary in Queensland and sequenced to evaluate bacterial community structure. We identified 25 core bacteria, most of which were present in male and female samples. Genera such as Fusobacterium, Bacteroides, Escherichia-Shigella, and Lactobacillus were consistently abundant, regardless of sex or gestation stage, accounting for 58.00% and 56.14% of reads in male and female samples, respectively. The echidna microbiome remained stable across the different gestation stages, though there was a significant difference in microbiota composition between male and female echidnas. This study is the first to describe the microbiome composition of short-beaked echidnas across reproductive phases and allows the opportunity for this novel information to be used as a metric of health to aid in the detection of diseases triggered by microbiota dysbiosis.

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来源期刊
MicrobiologyOpen
MicrobiologyOpen MICROBIOLOGY-
CiteScore
8.00
自引率
0.00%
发文量
78
审稿时长
20 weeks
期刊介绍: MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.
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