Production of DPP-IV inhibitory peptides-rich beta casein hydrolysates from milk of Gir (Bos indicus) cow and their evaluation for potential antidiabetic effect through in vitro assay

An attempt was made to produce hydrolysates from β-casein isolated from milk of Gir cow (Bos indicus species) using pepsin, trypsin, protease from Aspergillus oryzae (AOP) and proteinase-K. The AOP-treated hydrolysates exhibited highest DPP-IV inhibition (50.41 ± 1.44%) after 10 h hydrolysis at 4% E:S ratio. The resultant hydrolysates were characterised using RP-HPLC and LC–MS/MS and their peptides sequences identified. The efficacy of the low molecular weight hydrolysates (3 kDa permeate) was assessed through MIN6 cell line model at different doses. The dose of 30 mg/mL enhanced insulin production by ~51% and reduced the apoptosis by ~84%. The developed β-casein hydrolysates could be used as a dietary supplement to manage diabetes effectively.