[利用 AP-PCR、SSRP 和 SSR 检测栽培大豆的分子和遗传多态性]。

TSitologiia i genetika Pub Date : 2001-09-01
A F Brik, Iu M Sivolap
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引用次数: 0

摘要

利用三种 PCR 方法(AP-PCR、SSRP 和 ISSR)分析了不同地理区域栽培大豆的分子遗传多态性。根据获得的数据构建了表型遗传关系树枝图。品种分配与血统信息十分吻合。计算了多态性水平、预期杂合度和标记指数等主要参数。结果表明,ISSR 是区分大豆品种的首选方法。
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[Molecular and genetic polymorphism of cultivated soybean detected by AP-PCR, SSRP and SSR].

Molecular-genetic polymorphism of cultivated soybean from different geographic regions was analysed using three PCR methods (AP-PCR, SSRP and ISSR). The dendrogram of phenogenetic relationship was constructed on the base of obtained data. The allocation of varieties are in a good agreement with pedigree information. Main parameters such as polymorphism level, expected heterozygosity, and marker index were calculated. It was shown that ISSR is the method of choice for the differentiation of soybean varieties.

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