E A Trifonova, M L Komarova, O A Syrnik, A V Kochetov, V K Shumnyĭ
{"title":"[表达 Serratia marcescens 核酸酶编码基因的转基因烟草（Nicotiana tabacum SR1）植物]。","authors":"E A Trifonova, M L Komarova, O A Syrnik, A V Kochetov, V K Shumnyĭ","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The gene coding for the secreted Serratia marcescens endonuclease was fused with the mannopine synthase promoter of Agrobacterium tumefaciens Ti plasmid and transferred to Nicotiana tabacum SR1 plants. The promoter is leaf- and root-specific. The resulting transgenic plants demonstrated elevated nuclease activity. The level of the transgene product was determined in the transgenic lines.</p>","PeriodicalId":94019,"journal":{"name":"Genetika","volume":"38 2","pages":"274-7"},"PeriodicalIF":0.0000,"publicationDate":"2002-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease].\",\"authors\":\"E A Trifonova, M L Komarova, O A Syrnik, A V Kochetov, V K Shumnyĭ\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The gene coding for the secreted Serratia marcescens endonuclease was fused with the mannopine synthase promoter of Agrobacterium tumefaciens Ti plasmid and transferred to Nicotiana tabacum SR1 plants. The promoter is leaf- and root-specific. The resulting transgenic plants demonstrated elevated nuclease activity. The level of the transgene product was determined in the transgenic lines.</p>\",\"PeriodicalId\":94019,\"journal\":{\"name\":\"Genetika\",\"volume\":\"38 2\",\"pages\":\"274-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Genetika\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetika","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
将编码分泌型 Serratia marcescens 内切酶的基因与 Agrobacterium tumefaciens Ti 质粒的甘露碱合成酶启动子融合，并转移到烟草 SR1 植物上。该启动子具有叶和根特异性。由此产生的转基因植株表现出较高的核酸酶活性。对转基因品系中的转基因产物水平进行了测定。
[Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease].
The gene coding for the secreted Serratia marcescens endonuclease was fused with the mannopine synthase promoter of Agrobacterium tumefaciens Ti plasmid and transferred to Nicotiana tabacum SR1 plants. The promoter is leaf- and root-specific. The resulting transgenic plants demonstrated elevated nuclease activity. The level of the transgene product was determined in the transgenic lines.
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