开发三维灌注体外系统以评估化合物的促血管生成特性

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS Methods and Protocols Pub Date : 2023-12-09 DOI:10.3390/mps6060119
Johanna Alm, Benoit Fischer, Alexandra Emanuela Burger, Francesca Moretti
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引用次数: 0

摘要

血管生成受到干扰与多种疾病有关,人们正在积极探索促进和抗血管生成的疗法。此外,药物对血管生成的意外不良影响可能会导致肿瘤恶化和心血管并发症。与二维静态培养内皮细胞相比,三维微流控芯片血管系统能更准确地研究血管生理和病理。OrganoPlate® 片上血管生成系统已被证明可用于高通量筛选分子的抗血管生成特性。我们着手改造该系统,用于高通量筛选具有促血管生成特性的分子。我们对 OrganoPlate® 片上血管生成测定法的技术改进扩大了其适用范围,可用于早期筛选具有抗血管生成和促进血管生成特性的化合物,以便对血管生成进行治疗性调节,以及鉴定与血管生成相关的药物引起的血管毒性。
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Development of a 3D Perfused In Vitro System to Assess Proangiogenic Properties of Compounds.

Perturbation of angiogenesis is associated with a variety of diseases and pro- as well as antiangiogenic therapies are being actively explored. Additionally, unintended adverse drug effects on angiogenesis might lead to promotion of tumor progression and cardiovascular complications. Several tri-dimensional microfluidic vessel-on-chip systems have been described that allow a more accurate investigation of vascular physiology and pathology, compared to the two-dimensional static culture of endothelial cells. The OrganoPlate® angiogenesis-on-chip system has been demonstrated to be amenable to high-throughput screening for the antiangiogenic properties of molecules. We set out to adapt this system for high-throughput screening of molecules with proangiogenic properties. Our technical advancement of the OrganoPlate® angiogenesis-on-chip assay expands its applicability in the early screening of both anti- as well as proangiogenic properties of compounds for therapeutic modulation of angiogenesis as well as the identification of angiogenesis-associated drug-induced vascular toxicities.

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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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