{"title":"发夹延伸法是提高寡核苷酸探针灵敏度的常用方法","authors":"K.S. Sriprakash , J. Hartas","doi":"10.1016/0735-0651(89)90023-X","DOIUrl":null,"url":null,"abstract":"<div><p>A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3′ end a hairpin structure, allowing extension by polymerase reaction.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"6 2","pages":"Pages 29-32"},"PeriodicalIF":0.0000,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(89)90023-X","citationCount":"2","resultStr":"{\"title\":\"Hairpin extension a general method for the improvement of sensitivity of oligonucleotide probes\",\"authors\":\"K.S. Sriprakash , J. Hartas\",\"doi\":\"10.1016/0735-0651(89)90023-X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3′ end a hairpin structure, allowing extension by polymerase reaction.</p></div>\",\"PeriodicalId\":77714,\"journal\":{\"name\":\"Gene analysis techniques\",\"volume\":\"6 2\",\"pages\":\"Pages 29-32\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1989-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0735-0651(89)90023-X\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene analysis techniques\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/073506518990023X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073506518990023X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Hairpin extension a general method for the improvement of sensitivity of oligonucleotide probes
A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3′ end a hairpin structure, allowing extension by polymerase reaction.
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