重组瘦素蛋白对体外舌鳎 HPG 轴和肝脏关键基因表达的影响

IF 2.1 3区 农林科学 Q2 FISHERIES Fishes Pub Date : 2023-12-16 DOI:10.3390/fishes8120608
Xin Cai, Yaxing Zhang, Bin Wang, Aijun Cui, Yan Jiang, Zhaojun Meng, Yuting Li, Yongjiang Xu
{"title":"重组瘦素蛋白对体外舌鳎 HPG 轴和肝脏关键基因表达的影响","authors":"Xin Cai, Yaxing Zhang, Bin Wang, Aijun Cui, Yan Jiang, Zhaojun Meng, Yuting Li, Yongjiang Xu","doi":"10.3390/fishes8120608","DOIUrl":null,"url":null,"abstract":"Leptin (Lep) plays a crucial role in controlling food intake and maintaining energy balance in mammals. While several studies have shown the presence of multiple leptin genes in teleosts, limited information is currently available on how sex steroid hormones regulate the expression of these genes in fish. In this study, we used two previously expressed and purified leptin proteins to incubate four tissues in vitro (hypothalamic-pituitary-gonadal axis and liver) and used the RT-qPCR method to detect the expression of genes related to growth and reproduction in tongue sole (Cynoglossus semilaevis). The results showed that both recombinant LepA and LepB proteins of tongue sole almost entirely suppressed the expression of genes related to growth and reproduction in the hypothalamic-pituitary-gonadal axis and liver, while LepB may have had a positive role on steroid synthesis in gonads. Further, a high concentration of LepA facilitated the expression of IGF-Ι in liver. At the same time, we utilized human leptin to incubate four tissues in vitro; although most of them had trends similar to those stimulated with tongue sole leptins, there were still some differences, indicating differences among leptin homologs between humans and fishes. To our knowledge, this is the first study to explore the function of tongue sole LepA and LepB within the hypothalamic-pituitary-gonadal axis and liver in vitro. Our results provide a valuable resource and foundation for future studies.","PeriodicalId":12405,"journal":{"name":"Fishes","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2023-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of Recombinant Leptin Proteins on the Expression of Key Genes in the HPG Axis and Liver of Tongue Sole In Vitro\",\"authors\":\"Xin Cai, Yaxing Zhang, Bin Wang, Aijun Cui, Yan Jiang, Zhaojun Meng, Yuting Li, Yongjiang Xu\",\"doi\":\"10.3390/fishes8120608\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Leptin (Lep) plays a crucial role in controlling food intake and maintaining energy balance in mammals. While several studies have shown the presence of multiple leptin genes in teleosts, limited information is currently available on how sex steroid hormones regulate the expression of these genes in fish. In this study, we used two previously expressed and purified leptin proteins to incubate four tissues in vitro (hypothalamic-pituitary-gonadal axis and liver) and used the RT-qPCR method to detect the expression of genes related to growth and reproduction in tongue sole (Cynoglossus semilaevis). The results showed that both recombinant LepA and LepB proteins of tongue sole almost entirely suppressed the expression of genes related to growth and reproduction in the hypothalamic-pituitary-gonadal axis and liver, while LepB may have had a positive role on steroid synthesis in gonads. Further, a high concentration of LepA facilitated the expression of IGF-Ι in liver. At the same time, we utilized human leptin to incubate four tissues in vitro; although most of them had trends similar to those stimulated with tongue sole leptins, there were still some differences, indicating differences among leptin homologs between humans and fishes. To our knowledge, this is the first study to explore the function of tongue sole LepA and LepB within the hypothalamic-pituitary-gonadal axis and liver in vitro. Our results provide a valuable resource and foundation for future studies.\",\"PeriodicalId\":12405,\"journal\":{\"name\":\"Fishes\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-12-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fishes\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/fishes8120608\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fishes","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/fishes8120608","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

摘要

瘦素(Lep)在哺乳动物控制食物摄入量和维持能量平衡方面发挥着至关重要的作用。虽然多项研究表明远洋鱼类体内存在多个瘦素基因,但目前关于性类固醇激素如何调节这些基因在鱼类体内表达的信息十分有限。在本研究中,我们利用两种先前表达和纯化的瘦素蛋白对四种组织(下丘脑-垂体-性腺轴和肝脏)进行体外培养,并使用 RT-qPCR 方法检测舌鳎(Cynoglossus semilaevis)生长和繁殖相关基因的表达。结果表明,舌鳎的重组 LepA 和 LepB 蛋白几乎完全抑制了下丘脑-垂体-性腺轴和肝脏中与生长和繁殖有关的基因的表达,而 LepB 可能对性腺中类固醇的合成有积极作用。此外,高浓度的 LepA 能促进肝脏中 IGF-Ι 的表达。同时,我们利用人体瘦素对四种组织进行了体外培养,虽然大多数组织与舌鳎瘦素的刺激趋势相似,但仍存在一些差异,这表明人与鱼类的瘦素同源物之间存在差异。据我们所知,这是首次在体外探讨舌鳎瘦素 LepA 和 LepB 在下丘脑-垂体-性腺轴和肝脏中的功能。我们的研究结果为今后的研究提供了宝贵的资源和基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Effects of Recombinant Leptin Proteins on the Expression of Key Genes in the HPG Axis and Liver of Tongue Sole In Vitro
Leptin (Lep) plays a crucial role in controlling food intake and maintaining energy balance in mammals. While several studies have shown the presence of multiple leptin genes in teleosts, limited information is currently available on how sex steroid hormones regulate the expression of these genes in fish. In this study, we used two previously expressed and purified leptin proteins to incubate four tissues in vitro (hypothalamic-pituitary-gonadal axis and liver) and used the RT-qPCR method to detect the expression of genes related to growth and reproduction in tongue sole (Cynoglossus semilaevis). The results showed that both recombinant LepA and LepB proteins of tongue sole almost entirely suppressed the expression of genes related to growth and reproduction in the hypothalamic-pituitary-gonadal axis and liver, while LepB may have had a positive role on steroid synthesis in gonads. Further, a high concentration of LepA facilitated the expression of IGF-Ι in liver. At the same time, we utilized human leptin to incubate four tissues in vitro; although most of them had trends similar to those stimulated with tongue sole leptins, there were still some differences, indicating differences among leptin homologs between humans and fishes. To our knowledge, this is the first study to explore the function of tongue sole LepA and LepB within the hypothalamic-pituitary-gonadal axis and liver in vitro. Our results provide a valuable resource and foundation for future studies.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Fishes
Fishes Multiple-
CiteScore
1.90
自引率
8.70%
发文量
311
期刊最新文献
Effect of a Guar Meal Protein Concentrate in Replacement of Conventional Feedstuffs on Productive Performances and Gut Health of Rainbow Trout (Oncorhynchus mykiss) RTL-YOLOv8n: A Lightweight Model for Efficient and Accurate Underwater Target Detection Wetted Ramps Selectively Block Upstream Passage of Adult Sea Lampreys Practice of Territorial Use Rights in Fisheries in Coastal Fishery Management in China: A Case Study of the Island Reefs Fishery Lease Policy from Shengsi County in Zhejiang Province Characterization of Ovarian Lipid Composition in the Largemouth Bronze Gudgeon (Coreius guichenoti) at Different Development Stages
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1