温室水培甜瓜(Cucumis melo L.)枯萎病镰刀菌研究

Hendra Ardi Susanto, Achmad Himawan, E. N. Kristalisasi
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摘要

本研究旨在确定甜瓜植株上牛孢镰刀菌枯萎病的调查地点和症状、如何对出现牛孢镰刀菌枯萎病症状的植株进行取样、在实验室中分离牛孢镰刀菌真菌、将牛孢镰刀菌真菌孢子接种到甜瓜植株上、牛孢镰刀菌真菌孢子接种后的潜伏期和发病率、甜瓜果实重量和甜瓜果实净型。这项研究是在日惹特区斯莱曼县德波克区马古沃哈尔乔村的印度尼西亚拉汉温室(LI)进行的。研究时间为 2023 年 4 月至 6 月。研究采用描述性和定量方法对研究对象进行研究、描述、解释和说明。研究样本植物为 26 株,其中 5 株作为对照,21 株接种了 F. oxysporum 真菌孢子。调查地点为日惹省 Sleman 县 Kalasan 区 Selomartani 村 Plataran 温室。调查结果显示,有一些甜瓜植株出现了草孢镰刀菌枯萎病的症状。受影响的甜瓜植株占植株总数的 40%。在距离土壤表面 20 厘米处对出现 F. oxysporum 枯萎症状的甜瓜植株茎部进行取样,并剪成 10 厘米长。取 2 株出现 F. oxysporum 枯萎症状的植株。在培养皿中使用 PDA 培养基分离 F. oxysporum 真菌。发现真菌菌落和大孢子器,这是草孢子菌真菌孢子的特征,形状像新月。将真菌孢子接种到甜瓜植株茎上会导致健康植株生病。在第 10 天,有 2% 或 9% 的植株出现 F. oxysporum 枯萎症状。与试验植株相比,对照植株瓜果的重量和净形存在差异。
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Kajian Penyakit Layu Fusarium oxysporum pada Tanaman Melon (Cucumis melo L.) Hidroponik di Greenhouse
This research aims to determine the survey location and symptoms of F. oxysporum wilt on melon plants, how to take samples of plants with symptoms of F. oxysporum wilt, isolate the F. oxysporum fungus in the laboratory, inoculate F. oxysporum fungal spores onto melon plants, incubation period and percentage disease attacks after inoculation of F. oxysporum fungal spores, melon fruit weight and net pattern of melon fruit. This research was carried out at Greenhouse Lahan Indonesia (LI), Maguwoharjo Village, Depok District, Sleman Regency, Yogyakarta Special Region. The research was conducted from April to June 2023. The research used descriptive and quantitative methods to study, describe, explain and describe the objects studied. The research sample plants were 26 plants, namely 5 plants as controls, 21 plants were inoculated with F. oxysporum fungal spores. Location of survey for Fusarium oxysporum wilt symptoms in the Plataran Greenhouse, Selomartani Village, Kalasan District, Sleman Regency, DI Yogyakarta. The survey results showed that there were melon plants that had symptoms of F. oxysporum wilt. The percentage of melon plants affected is 40% of the total plant population. Sampling of melon plant stems with symptoms of F. oxysporum wilting was taken 20 cm from the soil surface and cut to a length of 10 cm. Take 2 plants that experience symptoms of F. oxysporum wilt. Isolation of F. oxysporum fungus using PDA media in a petri dish. Fungal colonies and macroconidia were found which are characteristic of F. oxysporum fungal spores which are shaped like a crescent moon. Inoculation of fungal spores on melon plant stems can cause healthy plants to become sick. On the 10th day there were 2 or 9% of plants showing symptoms of F. oxysporum wilting. There were differences in the weight and net pattern of melon fruit on control plants compared to test plants.
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