加速醋酸生物生产:作为可持续生物商品生产者的产酸菌

IF 3.4 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Current Opinion in Systems Biology Pub Date : 2023-12-12 DOI:10.1016/j.coisb.2023.100500
Maximilian Flaiz , Diana Z. Sousa
{"title":"加速醋酸生物生产:作为可持续生物商品生产者的产酸菌","authors":"Maximilian Flaiz ,&nbsp;Diana Z. Sousa","doi":"10.1016/j.coisb.2023.100500","DOIUrl":null,"url":null,"abstract":"<div><p>Gas fermentation using autotrophic acetogenic bacteria has been industrialized, however, its full potential remains untapped, with only native products like ethanol being produced thus far. Advancements in synthetic biology have enabled the recombinant production of diverse biocommodities to broaden their limited natural product spectrum from C1-gases. Additionally, co-culturing acetogens with other microorganisms holds the potential for expanding the product spectrum further. However, commercialization remains challenging due to complex pathway and (co)culturing optimizations. To address this, novel synthetic biology tools, including the use of high throughput biopart screenings using reporter proteins, the deployment of cell-free systems to combine best-performing enzymes, and the identification and elimination of competing pathways, can be employed. Incorporating genetically engineered strains in co-cultures improves dependencies, directs product formation, and increases resilience, enhancing bioproduction efficiency. This review emphasizes using these tools to enhance the recombinant production of biocommodities, offering promising solutions to overcome existing challenges.</p></div>","PeriodicalId":37400,"journal":{"name":"Current Opinion in Systems Biology","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2452310023000574/pdfft?md5=b34324e70ac5822168b823c24989c3e6&pid=1-s2.0-S2452310023000574-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Accelerate acetogenic bioproduction: Acetogens as sustainable producers of biocommodities\",\"authors\":\"Maximilian Flaiz ,&nbsp;Diana Z. Sousa\",\"doi\":\"10.1016/j.coisb.2023.100500\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Gas fermentation using autotrophic acetogenic bacteria has been industrialized, however, its full potential remains untapped, with only native products like ethanol being produced thus far. Advancements in synthetic biology have enabled the recombinant production of diverse biocommodities to broaden their limited natural product spectrum from C1-gases. Additionally, co-culturing acetogens with other microorganisms holds the potential for expanding the product spectrum further. However, commercialization remains challenging due to complex pathway and (co)culturing optimizations. To address this, novel synthetic biology tools, including the use of high throughput biopart screenings using reporter proteins, the deployment of cell-free systems to combine best-performing enzymes, and the identification and elimination of competing pathways, can be employed. Incorporating genetically engineered strains in co-cultures improves dependencies, directs product formation, and increases resilience, enhancing bioproduction efficiency. This review emphasizes using these tools to enhance the recombinant production of biocommodities, offering promising solutions to overcome existing challenges.</p></div>\",\"PeriodicalId\":37400,\"journal\":{\"name\":\"Current Opinion in Systems Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2023-12-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2452310023000574/pdfft?md5=b34324e70ac5822168b823c24989c3e6&pid=1-s2.0-S2452310023000574-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Opinion in Systems Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452310023000574\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Opinion in Systems Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452310023000574","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

利用自养醋酸菌进行气体发酵已经实现了工业化,但其全部潜力仍未开发,迄今只能生产乙醇等本地产品。合成生物学的进步使得重组生产多种生物商品成为可能,从而扩大了 C1 气体的有限天然产品范围。此外,与其他微生物共同培养乙炔原也有可能进一步扩大产品范围。然而,由于复杂的途径和(共)培养优化,商业化仍具有挑战性。为解决这一问题,可采用新型合成生物学工具,包括使用报告蛋白进行高通量生物部分筛选、部署无细胞系统以组合性能最佳的酶,以及识别和消除竞争途径。在共培养物中加入基因工程菌株可改善依赖性、指导产品的形成并提高复原力,从而提高生物生产效率。本综述强调利用这些工具来提高生物商品的重组生产,为克服现有挑战提供有前景的解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Accelerate acetogenic bioproduction: Acetogens as sustainable producers of biocommodities

Gas fermentation using autotrophic acetogenic bacteria has been industrialized, however, its full potential remains untapped, with only native products like ethanol being produced thus far. Advancements in synthetic biology have enabled the recombinant production of diverse biocommodities to broaden their limited natural product spectrum from C1-gases. Additionally, co-culturing acetogens with other microorganisms holds the potential for expanding the product spectrum further. However, commercialization remains challenging due to complex pathway and (co)culturing optimizations. To address this, novel synthetic biology tools, including the use of high throughput biopart screenings using reporter proteins, the deployment of cell-free systems to combine best-performing enzymes, and the identification and elimination of competing pathways, can be employed. Incorporating genetically engineered strains in co-cultures improves dependencies, directs product formation, and increases resilience, enhancing bioproduction efficiency. This review emphasizes using these tools to enhance the recombinant production of biocommodities, offering promising solutions to overcome existing challenges.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Current Opinion in Systems Biology
Current Opinion in Systems Biology Mathematics-Applied Mathematics
CiteScore
7.10
自引率
2.70%
发文量
20
期刊介绍: Current Opinion in Systems Biology is a new systematic review journal that aims to provide specialists with a unique and educational platform to keep up-to-date with the expanding volume of information published in the field of Systems Biology. It publishes polished, concise and timely systematic reviews and opinion articles. In addition to describing recent trends, the authors are encouraged to give their subjective opinion on the topics discussed. As this is such a broad discipline, we have determined themed sections each of which is reviewed once a year. The following areas will be covered by Current Opinion in Systems Biology: -Genomics and Epigenomics -Gene Regulation -Metabolic Networks -Cancer and Systemic Diseases -Mathematical Modelling -Big Data Acquisition and Analysis -Systems Pharmacology and Physiology -Synthetic Biology -Stem Cells, Development, and Differentiation -Systems Biology of Mold Organisms -Systems Immunology and Host-Pathogen Interaction -Systems Ecology and Evolution
期刊最新文献
From regulation of cell fate decisions towards patient-specific treatments, insights from mechanistic models of signalling pathways Editorial overview: Systems biology of ecological interactions across scales A critical review of multiscale modeling for predictive understanding of cancer cell metabolism Network modeling approaches for metabolic diseases and diabetes Contents
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1