Muhammad Munir Khan, Syed Muhammad Suhail, Hafiz Abdul Majid, Ijaz Ahmad, Umer Sadique, Rajwali Khan, Iftikhar Ahmad, Asim Ijaz, Khalid Khan, Farhad Ali, Muhammad Saeed Khan, Ahmed A El-Mansi
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引用次数: 0
摘要
本研究旨在通过线粒体 DNA 区域,即细胞色素 C 氧化酶亚基一(CO1)基因,研究库哈特(Kohat)地区当地苏古利山羊的形态特征以及系统发育和多样性。采集了三十(30)只纯种山羊的形态数据和血液样本。形态分析表明,性别对其他特征有显著影响(p p > 0.05)。结果还表明,年龄对其他特征也有显著影响(p p > 0.05)。通过核苷酸范围为 1-767 的 CO1 核苷酸序列进行的系统进化分析表明,9 个多态位点分离成 8 个单倍型。计算得出的种内平均多样性和种间平均多样性分别为 0.23% 和 2.36%。系统发育树显示,山羊和本地苏尔古丽山羊有共同的祖先。本研究获得的形态计量学和分子结果可作为育种和整体改良的选育工具。
Morpometric and molecular characterization of Surguli goat through CO1 gene in district Kohat.
The present study was designed with the aim to study morphometric characterization as well as phylogeny and diversity of the local Surguli goat at their breeding tract district Kohat through mitochondrial DNA region, i.e., Cytochrome C Oxidase Subunit One (CO1) gene. Morphometric data and blood samples were collected from thirty (30) pure goats. Morphometric analysis showed that sex had significant effect (p < 0.05) on body weight, body length, hearth girth and horn length while no significant effect (p > 0.05) was observed for other characteristics. The results also indicated that age had significant effect (p < 0.05) on height at rump, ear length, horn length and tail length while no significant effect (p > 0.05) was observed for other characteristics. The phylogenetic analysis through CO1 nucleotide sequences within nucleotide range 1-767 showed nine polymorphic sites segregating into eight haplotypes. The mean intraspecific diversity and mean interspecific diversity were calculated as 0.23 and 2.36%, respectively. Phylogenetic tree revealed that Capra Ibex and native Surguli goat have common ancestors. The morphometric and molecular results obtained from the present study can be exploited as a selection tool for breeding and overall improvement.
期刊介绍:
Biotechnology can be defined as any technique that uses living organisms (or parts of organisms like cells, genes, proteins) to make or modify products, to improve plants, animals or microorganisms for a specific use. Animal Biotechnology publishes research on the identification and manipulation of genes and their products, stressing applications in domesticated animals. The journal publishes full-length articles and short research communications, as well as comprehensive reviews. The journal also provides a forum for regulatory or scientific issues related to cell and molecular biology applied to animal biotechnology.
Submissions on the following topics are particularly welcome:
- Applied microbiology, immunogenetics and antibiotic resistance
- Genome engineering and animal models
- Comparative genomics
- Gene editing and CRISPRs
- Reproductive biotechnologies
- Synthetic biology and design of new genomes