{"title":"PMab-301：一种用于免疫组化的抗吉拉夫波多波宁单克隆抗体。","authors":"Tsunenori Ouchida, Tomohiro Tanaka, Hiroyuki Suzuki, Kazuyuki Uchida, Takayuki Nakagawa, Guanjie Li, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Mika K Kaneko, Yukinari Kato","doi":"10.1089/mab.2023.0020","DOIUrl":null,"url":null,"abstract":"<p><p>Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG<sub>1</sub>, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.</p>","PeriodicalId":53514,"journal":{"name":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry.\",\"authors\":\"Tsunenori Ouchida, Tomohiro Tanaka, Hiroyuki Suzuki, Kazuyuki Uchida, Takayuki Nakagawa, Guanjie Li, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Mika K Kaneko, Yukinari Kato\",\"doi\":\"10.1089/mab.2023.0020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG<sub>1</sub>, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.</p>\",\"PeriodicalId\":53514,\"journal\":{\"name\":\"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1089/mab.2023.0020\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/26 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Monoclonal Antibodies in Immunodiagnosis and Immunotherapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1089/mab.2023.0020","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/26 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
免疫组化染色是病理诊断的重要方法。Podoplanin（PDPN）是肺泡上皮、淋巴管和肾小球的特异性制造者。在这项研究中，我们利用基于细胞的免疫和筛选（CBIS）方法建立了一种新型抗吉拉夫 PDPN（girPDPN） mAb--PMab-301。PMab-301（小鼠 IgG1，卡帕型）可在流式细胞术、Western 印迹和免疫组化等多种应用中检测到 girPDPN。使用福尔马林固定的石蜡包埋的长颈鹿肺组织，PMab-301 能特异性染色 I 型肺泡细胞。我们的研究结果表明，PMab-301 在长颈鹿组织的病理生理学分析中具有潜在的用途。
PMab-301: An Anti-Giraffe Podoplanin Monoclonal Antibody for Immunohistochemistry.
Immunohistochemistry staining is an essential method in pathological diagnoses. Podoplanin (PDPN) is a specific maker of alveolar epithelium, lymphatic vessels, and glomeruli. In this study, we established a novel anti-giraffe PDPN (girPDPN) mAb, PMab-301, using the Cell-Based Immunization and Screening (CBIS) method. PMab-301 (mouse IgG1, kappa) detected girPDPN in various applications, such as flow cytometry, western blot, and immunohistochemistry. PMab-301 specifically stained type-I alveolar cells using formalin-fixed paraffin-embedded giraffe lung tissues. Our findings suggest the potential usefulness of PMab-301 for the pathophysiological analyses of giraffe tissues.
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