在动脉粥样硬化的诱导性小鼠模型中,暴露于电子烟会导致早期促动脉粥样硬化变化。

IF 3.6 Q2 TOXICOLOGY Frontiers in toxicology Pub Date : 2023-12-18 eCollection Date: 2023-01-01 DOI:10.3389/ftox.2023.1244596
Bayan Alakhtar, Cynthia Guilbert, Nivetha Subramaniam, Vincenza Caruana, Kiran Makhani, Carolyn J Baglole, Koren K Mann
{"title":"在动脉粥样硬化的诱导性小鼠模型中,暴露于电子烟会导致早期促动脉粥样硬化变化。","authors":"Bayan Alakhtar, Cynthia Guilbert, Nivetha Subramaniam, Vincenza Caruana, Kiran Makhani, Carolyn J Baglole, Koren K Mann","doi":"10.3389/ftox.2023.1244596","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction:</b> Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Moreover, there is a poor understanding of how risk factors for atherosclerosis (i.e., hyperlipidemia, high-fat diet) intersect with vaping to promote disease risk. Herein, we evaluated whether there was early evidence of atherosclerosis in an inducible hyperlipidemic mouse exposed to aerosol from commercial pod-style devices and e-liquid. <b>Methods:</b> Mice were injected with adeno-associated virus containing the human protein convertase subtilisin/kexin type 9 (PCSK9) variant to promote hyperlipidemia. These mice were fed a high-fat diet and exposed to room air or aerosol derived from JUUL pods containing polyethylene glycol/vegetable glycerin (PG/VG) or 5% nicotine with mango flavoring for 4 weeks; this timepoint was utilized to assess markers of atherosclerosis that may occur prior to the development of atherosclerotic plaques. <b>Results:</b> These data show that various parameters including weight, circulating lipoprotein/glucose levels, and splenic immune cells were significantly affected by exposure to PG/VG and/or nicotine-containing aerosols. <b>Discussion:</b> Not only can this mouse model be utilized for chronic vaping studies to assess the vascular pathology but these data support that vaping is not risk-free and may increase CVD outcomes later in life.</p>","PeriodicalId":73111,"journal":{"name":"Frontiers in toxicology","volume":"5 ","pages":"1244596"},"PeriodicalIF":3.6000,"publicationDate":"2023-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757938/pdf/","citationCount":"0","resultStr":"{\"title\":\"E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis.\",\"authors\":\"Bayan Alakhtar, Cynthia Guilbert, Nivetha Subramaniam, Vincenza Caruana, Kiran Makhani, Carolyn J Baglole, Koren K Mann\",\"doi\":\"10.3389/ftox.2023.1244596\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction:</b> Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Moreover, there is a poor understanding of how risk factors for atherosclerosis (i.e., hyperlipidemia, high-fat diet) intersect with vaping to promote disease risk. Herein, we evaluated whether there was early evidence of atherosclerosis in an inducible hyperlipidemic mouse exposed to aerosol from commercial pod-style devices and e-liquid. <b>Methods:</b> Mice were injected with adeno-associated virus containing the human protein convertase subtilisin/kexin type 9 (PCSK9) variant to promote hyperlipidemia. These mice were fed a high-fat diet and exposed to room air or aerosol derived from JUUL pods containing polyethylene glycol/vegetable glycerin (PG/VG) or 5% nicotine with mango flavoring for 4 weeks; this timepoint was utilized to assess markers of atherosclerosis that may occur prior to the development of atherosclerotic plaques. <b>Results:</b> These data show that various parameters including weight, circulating lipoprotein/glucose levels, and splenic immune cells were significantly affected by exposure to PG/VG and/or nicotine-containing aerosols. <b>Discussion:</b> Not only can this mouse model be utilized for chronic vaping studies to assess the vascular pathology but these data support that vaping is not risk-free and may increase CVD outcomes later in life.</p>\",\"PeriodicalId\":73111,\"journal\":{\"name\":\"Frontiers in toxicology\",\"volume\":\"5 \",\"pages\":\"1244596\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2023-12-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757938/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3389/ftox.2023.1244596\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3389/ftox.2023.1244596","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"TOXICOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

导言:有证据表明,使用电子烟(吸食电子烟)会增加心血管疾病的风险,但吸食电子烟的人还需要数十年才会出现病变。因此,动脉粥样硬化的小鼠模型可用作了解疾病易感性、风险和发病机制的工具。此外,人们对动脉粥样硬化的危险因素(即高脂血症、高脂饮食)如何与吸食电子烟交织在一起以促进疾病风险还缺乏了解。在此,我们评估了一只诱导性高脂血症小鼠暴露于来自商用豆荚式装置和电子液体的气溶胶中是否有动脉粥样硬化的早期证据。研究方法给小鼠注射含有人蛋白枯草酶/kexin 9 型(PCSK9)变体的腺相关病毒,以促进高脂血症。给这些小鼠喂食高脂肪饮食,并将其暴露于室内空气或由含有聚乙二醇/植物甘油(PG/VG)或5%尼古丁与芒果香精的JUUL豆荚产生的气溶胶中4周;利用这个时间点来评估动脉粥样硬化斑块形成前可能出现的动脉粥样硬化标志物。结果这些数据表明,体重、循环脂蛋白/葡萄糖水平和脾脏免疫细胞等各种参数受到接触 PG/VG 和/或含尼古丁气溶胶的显著影响。讨论:这种小鼠模型不仅可用于慢性吸烟研究以评估血管病理学,而且这些数据还支持吸烟并非无风险,可能会增加日后心血管疾病的发病率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
E-cigarette exposure causes early pro-atherogenic changes in an inducible murine model of atherosclerosis.

Introduction: Evidence suggests that e-cigarette use (vaping) increases cardiovascular disease risk, but decades are needed before people who vape would develop pathology. Thus, murine models of atherosclerosis can be utilized as tools to understand disease susceptibility, risk and pathogenesis. Moreover, there is a poor understanding of how risk factors for atherosclerosis (i.e., hyperlipidemia, high-fat diet) intersect with vaping to promote disease risk. Herein, we evaluated whether there was early evidence of atherosclerosis in an inducible hyperlipidemic mouse exposed to aerosol from commercial pod-style devices and e-liquid. Methods: Mice were injected with adeno-associated virus containing the human protein convertase subtilisin/kexin type 9 (PCSK9) variant to promote hyperlipidemia. These mice were fed a high-fat diet and exposed to room air or aerosol derived from JUUL pods containing polyethylene glycol/vegetable glycerin (PG/VG) or 5% nicotine with mango flavoring for 4 weeks; this timepoint was utilized to assess markers of atherosclerosis that may occur prior to the development of atherosclerotic plaques. Results: These data show that various parameters including weight, circulating lipoprotein/glucose levels, and splenic immune cells were significantly affected by exposure to PG/VG and/or nicotine-containing aerosols. Discussion: Not only can this mouse model be utilized for chronic vaping studies to assess the vascular pathology but these data support that vaping is not risk-free and may increase CVD outcomes later in life.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.80
自引率
0.00%
发文量
0
审稿时长
13 weeks
期刊最新文献
Considering future qualification for regulatory science in the early development of microphysiological systems: a case study of microthrombosis in a Vessel-on-Chip. Protective effects of Pelargonium graveolens (geranium) oil against cefotaxime-induced hepato-renal toxicity in rats. The incorporation of MALDI mass spectrometry imaging in studies to identify markers of toxicity following in utero opioid exposures in mouse fetuses. In vitro toxicological evaluation of pouched portioned oral nicotine products. Heterogenous bioluminescence patterns, cell viability, and biofilm formation of Photobacterium leiognathi strains exposed to ground microplastics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1