[让看不见的变为看得见的--基于单一荧光蛋白的指示器的创新]。

Marie Mita, Tetsuya Kitaguchi
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引用次数: 0

摘要

生物现象是由蛋白质、代谢物、信号分子和离子等各种生物分子之间的合作和层次关系产生的。然而,在许多情况下,这些生物分子没有颜色,很难观察到它们的原貌。因此,有必要用颜色或荧光将每个分子 "可视化",并分析它们之间的功能关系。使用单荧光蛋白(FP)指示剂的活细胞成像技术为生物分子的可视化做出了贡献。基于单个荧光蛋白(FP)的指示剂在与目标分子结合后会改变其荧光强度,通过一系列创新的筛选方法,这些指示剂已被革新为多色指示剂。另一方面,我们利用半理性分子设计和分子进化建立了一种独创的筛选方法,并针对 cAMP 和葡萄糖等多种分子开发了多种基于单 FP 的指示剂。本文将重点介绍基于单一 FP 的指示剂,并介绍其开发策略和筛选方法的发展历程。
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[Make the invisible visible-innovation in the single fluorescent protein-based indicators].

Biological phenomena are generated by the cooperative and hierarchical relationships between a variety of biomolecules, such as proteins, metabolites, signaling molecules, and ions. In many cases, however, these biomolecules do not have color, and it is difficult to observe them as they are. Therefore, it is necessary to "visualize" each molecule with color or fluorescence, and to analyze the functional relationships between them. The live cell imaging technology using single fluorescent protein (FP)-based indicators has contributed to the visualization of biomolecules. Single FP-based indicators, which change their fluorescence intensity upon binding to the target molecule, have been revolutionized into multicolor indicators by a series of innovative screening methods. On the other hand, we have established an original screening method using semi-rational molecular design and molecular evolution, and have developed many single FP-based indicators for various molecules such as cAMP and glucose. In this article, we focus on single FP-based indicators and introduce their development strategy and the history of screening method.

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来源期刊
Folia Pharmacologica Japonica
Folia Pharmacologica Japonica Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
0.40
自引率
0.00%
发文量
132
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