{"title":"土耳其东南部收容所犬只中十二指肠贾第虫的流行率和基因型。","authors":"Burçak Aslan Çelik, Özgür Yaşar Çelik, Akın Koçhan, Adnan Ayan, Özlem Orunç Kılınç, Gürkan Akyıldız, Kıvanç İrak, Özge Oktay Ayan, Kerem Ercan","doi":"10.30466/vrf.2023.1998950.3826","DOIUrl":null,"url":null,"abstract":"<p><p><i>Giardia duodenalis</i> is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of <i>G. duodenalis</i> in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of <i>Giardia</i> as well as its possible role in the transmission of this parasite to humans.</p>","PeriodicalId":23989,"journal":{"name":"Veterinary Research Forum","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10758005/pdf/","citationCount":"0","resultStr":"{\"title\":\"Prevalence and genotypes of <i>Giardia duodenalis</i> in shelter dogs of southeastern Türkiye.\",\"authors\":\"Burçak Aslan Çelik, Özgür Yaşar Çelik, Akın Koçhan, Adnan Ayan, Özlem Orunç Kılınç, Gürkan Akyıldız, Kıvanç İrak, Özge Oktay Ayan, Kerem Ercan\",\"doi\":\"10.30466/vrf.2023.1998950.3826\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Giardia duodenalis</i> is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of <i>G. duodenalis</i> in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of <i>Giardia</i> as well as its possible role in the transmission of this parasite to humans.</p>\",\"PeriodicalId\":23989,\"journal\":{\"name\":\"Veterinary Research Forum\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10758005/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary Research Forum\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.30466/vrf.2023.1998950.3826\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ZOOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary Research Forum","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.30466/vrf.2023.1998950.3826","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
十二指肠贾第虫是一种原生动物寄生虫，存在于人类和几种哺乳动物体内。这种寄生虫遍布全球，被普遍认为是一种人畜共患病，据报道是导致人类和动物腹泻的最常见原因之一。本研究旨在确定位于土耳其东南部安纳托利亚地区迪亚巴克尔省的收容犬中 G. duodenalis 的流行率和基因型。对 100 份粪便样本进行的原生-卢戈尔法和巢式聚合酶链反应分析表明，患病率分别为 3.00% 和 4.00%。女性和 1 岁以下儿童的感染率较高。序列分析表明存在人畜共患的 B 型、D 型和 E 型组合。在这项研究中检测到人畜共患的 B 型组合，这表明狗可能是人类贾第虫病的储库。需要进一步开展分子研究，以确定贾第虫的基因型多样性及其在这种寄生虫向人类传播过程中可能发挥的作用。
Prevalence and genotypes of Giardia duodenalis in shelter dogs of southeastern Türkiye.
Giardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakır province being located in the southeastern Anatolia region of Türkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.
期刊介绍：
Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.
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