Lesley Sitter, Marion Schoof, Travis R. Glare, Murray P. Cox, Peter C. Fineran, Paul P. Gardner, Mark R. H. Hurst
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引用次数: 0
摘要
这篇文章描述了在感染甲虫幼虫 Costelytra giveni 的致病性沙雷氏菌中发现的与 ABC 毒素复合物(Tc)始终位于一起的 Sef 拟缘体的特征。在任何已知的 Tc 系统中,都没有记录过缘膜与 Tc 之间的这种独特联系,这就提出了一个问题:只有在编码 Tc 的细菌中才能维持这些缘膜的高选择压力。我们的研究还试图利用生物信息学、克隆和实验方法来描述 Sef 纤膜的结构和功能。
Serratia-based toxin cluster elements are associated with a type I fimbria
A soil bacterium in the Serratia genus, carrying a 153 kb conjugative amber disease-associated plasmid (pADAP), is commercially exploited for population control of the New Zealand endemic pest beetle Costelytra giveni (Coleoptera: Scarabaeidae). The main insecticidal elements are an anti-feeding prophage and the Sep ABC toxin complex (Tc). Homologs of pADAP, encoding variant Tcs, convey different beetle disease phenotypes. To investigate the correlation between variable bioactivity and the Tc variant, 76 Serratia plasmids were sequenced, resulting in the identification of four additional tc variants. All Serratia tc variants were found to be colocated with a conserved type 1 sef fimbrial-like operon, indicating a conserved sef-tc genetic island not observed outside of the Serratia genus. The conserved co-location of the fimbrial and tc genes suggests the fimbriae somehow contribute to the lifestyle of Tc-producing cells. Expression of the sef operon in a fim-null Escherichia coli strain revealed fimbriae presence while a constructed sef-deficient mutant showed no reduction of virulence or host colonization. Although no detectable contribution of Sef to amber disease in C. giveni was observed, the Sef adhesin sequences clustered similarly to the Serratia species encoding it, suggesting Sef has a species-specific function.
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