Assessment of radiation sensitivity and regeneration in shoot tip culture of banana cv. Grand Naine

An investigation was carried out to initiate in vitro mutagenesis in banana cv. Grand Naine shoot tip culture. Based on the sensitivity of explants, the lethal dose was estimated using probit analysis. The LD50 dose of gamma irradiation of the banana shoot tip culture was determined to be 39.5 Gy. It was also discovered that the number of new shoots rose with the number of subculture days, with the largest number of shoots recorded at 40 Gy. The study also discovered that when 13 DAS explants were treated with 10 Gy, they recovered the greatest number of shoots. As a result of the negative effects of higher doses of irradiation, it was decided that determining the lethal dose can be very beneficial in reducing population loss. Its further optimization may be effective in developing significant variations with a certain trait of interest.