两种 H3K36 甲基转移酶与稻瘟病真菌的转录活性和变异异染色质的富集有不同关系

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY aBIOTECH Pub Date : 2023-12-18 DOI:10.1007/s42994-023-00127-3
Mengting Xu, Ziyue Sun, Huanbin Shi, Jiangnan Yue, Xiaohui Xiong, Zhongling Wu, Yanjun Kou, Zeng Tao
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引用次数: 0

摘要

组蛋白 H3 上赖氨酸 36 的二甲基化和三甲基化(H3K36me2/3)是由组蛋白甲基转移酶 Set2 催化的,它在转录调控中发挥着重要作用。虽然在酵母和高等真核生物中只有一种 H3K36 甲基转移酶,但在许多丝状真菌中存在两种 H3K36 甲基转移酶,即 Ash1 和 Set2。然而,它们在 H3K36 甲基化和转录调控中的作用仍不清楚。结合RNA-seq和ChIP-seq方法,我们发现Ash1和Set2都是导致全球毁灭性稻瘟病的Magnaporthe oryzae中H3K36me2/3全部活性的冗余必需基因。Ash1和Set2可区分基因组H3K36me2/3标记区域,并分别与抑制转录和激活转录有不同的关联。此外,Ash1催化的H3K36me2与染色质中的H3K27me3共定位,H3K27me3占据基因的富集和转录沉默需要Ash1。由于Ash1和Set2在应激反应基因的H3K36me2/3富集和转录调控中的作用不同,它们对M. oryzae的各种应激反应也不同。总之,我们揭示了一种新的机制,即两种 H3K36 甲基转移酶催化 H3K36me2/3 与转录活动有不同的关联,并有助于真核生物中侧异染色质的富集。
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Two H3K36 methyltransferases differentially associate with transcriptional activity and enrichment of facultative heterochromatin in rice blast fungus

Di- and tri-methylation of lysine 36 on histone H3 (H3K36me2/3) is catalysed by histone methyltransferase Set2, which plays an essential role in transcriptional regulation. Although there is a single H3K36 methyltransferase in yeast and higher eukaryotes, two H3K36 methyltransferases, Ash1 and Set2, were present in many filamentous fungi. However, their roles in H3K36 methylation and transcriptional regulation remained unclear. Combined with methods of RNA-seq and ChIP-seq, we revealed that both Ash1 and Set2 are redundantly required for the full H3K36me2/3 activity in Magnaporthe oryzae, which causes the devastating worldwide rice blast disease. Ash1 and Set2 distinguish genomic H3K36me2/3-marked regions and are differentially associated with repressed and activated transcription, respectively. Furthermore, Ash1-catalysed H3K36me2 was co-localized with H3K27me3 at the chromatin, and Ash1 was required for the enrichment and transcriptional silencing of H3K27me3-occupied genes. With the different roles of Ash1 and Set2, in H3K36me2/3 enrichment and transcriptional regulation on the stress-responsive genes, they differentially respond to various stresses in M. oryzae. Overall, we reveal a novel mechanism by which two H3K36 methyltransferases catalyze H3K36me2/3 that differentially associate with transcriptional activities and contribute to enrichment of facultative heterochromatin in eukaryotes.

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