罗勒草提取物用于治疗万年青病和副溶血性弧菌的感染

H. Harlina, Muhammad Saenong, S. Syahrul, Andi Hamdillah, Inda Safitri Tangdilintin
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引用次数: 0

摘要

本研究旨在治疗感染副溶血性弧菌的凡纳滨对虾病。在这项研究中,我们采用了完全随机设计(CRD),4 个处理,3 次重复。测试的处理方法是用不同浓度的罗勒(O. basilicum)叶提取物浸泡香兰虾病,即处理 A(0 毫克/毫升)和处理 B(0 毫克/毫升):处理 A(0 毫克/毫升)、处理 B(1.56 毫克/毫升)、处理 C(3.12 毫克/毫升)和处理 D(12.5 毫克/毫升)。观察的参数是瓦纳米对虾的培养基和肝胰腺中副溶血病毒细菌菌落的数量。此外,还观察了万年青的存活率和水质参数。研究结果发现,治疗万年青对虾副溶血病毒病的最佳浓度为 12.5 毫克/毫升,饲养培养基中的细菌菌落数最少,为 1.20 x 102 CFU/毫升。在凡纳滨对虾的肝胰腺中,处理浓度为 12.5 毫克/毫升时,菌落数最少,为 1.12 x 102 CFU/毫升。处理浓度为 12.5 mg/ml 时,存活率最高,为 86.67±5.77%。研究观察中获得的水质范围如下:盐度 28.72-29.05 ppt,温度 26.71-27.25OC ,氧气范围 5.72-7.81 ,pH 值 7.18-8.13 ,氨氮 0.033-0.041ppm 。研究期间的水质符合凡纳滨对虾生活的最佳范围。
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The Use of (O. Basilicum) Extract to Treat Litopenaeus vannamei Disease the Infected Bacteria Vibrio parahaemolyticus
This research aims to treat vaname shrimp disease  infected with bacteriaVibrio parahaemolyticus. In this research, we used Completely Randomized Design (CRD) 4 treatments and 3 replications. The treatment being tested was the treatment of vaname shrimp disease by soaking in basil (O. basilicum) leaf extract with different concentrations, namely: Treatment A (0 mg/ml), treatment B (1.56 mg/ml), treatment C (3.12 mg/ml) and treatment D (12.5 mg/ml). The parameter observed was the number of bacterial coloniesV. parahaemolyticus maintenance media and in the hepatopancreas of vaname shrimp. Apart from that, observations were made on the survival rate of vaname shrimp and water quality parameters. The research results found that the best concentration in the treatment of diseaseV. parahaemolyticus in vaname shrimp at a concentration of 12.5 mg/ml with the lowest number of bacterial colonies in the rearing media, namely 1.20 x 102 CFU/ml. In the hepatopancreas of vaname shrimp, the lowest number of colonies was obtained at a treatment concentration of 12.5 mg/ml, namely 1.12 x 102 CFU/ml. The highest survival rate was obtained at a treatment concentration of 12.5 mg/ml of 86,67±5,77%. The range of water quality in research observations was obtained as follows salinity 28.72-29.05 ppt, temperature 26.71- 27.25OC, oxygen ranges from 5.72-7.81, pH 7.18-8.13 and ammonia 0.033-0.041ppm. Water quality during the research was in the optimal range for the life of vaname shrimp.
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