多酚高良姜素诱导 ROS 和 ER 应激介导的人结肠癌 HCT-116 细胞内在和外在凋亡途径

IF 0.7 4区 农林科学 Q3 AGRONOMY Emirates Journal of Food and Agriculture Pub Date : 2023-12-12 DOI:10.9755/ejfa.2023.3192
Ina Ramírez Miranda, David Betancur Ancona, Yolanda Moguel Ordóñez
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引用次数: 0

摘要

Melipona beecheii 蜂蜜中的生物活性化合物具有很高的抗氧化活性,会影响其颜色。这项研究评估了酚类化合物和类黄酮对 Melipona beecheii 蜂蜜颜色和抗氧化活性的影响。研究人员在采收季节和采收后季节(2020-2021 年)从墨西哥尤卡坦低矮落叶林的不同地点提取了 43 份蜂蜜样品。采用 Pfund 法测量颜色,Folin-Ciocalteu 法测量酚类,氯化铝法测定黄酮类化合物,ABTS 和 DPPH IC50 法测定抗氧化剂。两季蜂蜜的主要颜色为特浅琥珀色和浅琥珀色。酚类介于 780 至 1317 毫克/千克 GAE 之间;类黄酮介于 36 至 55 毫克/千克 CE 之间;ABTS 法测定的抗氧化剂为 675 至 1161 微摩尔/千克 TEAC,DPPH IC50 法测定的抗氧化剂为 361.8 至 173.0 毫克/毫升。 酚类和类黄酮与颜色和自由基清除活性相关,表明这些化合物会影响蜂蜜的颜色和抗氧化活性。不同季节之间的差异(P<0.05)具有统计学意义,这可能与尤卡坦半岛低矮落叶林的植物多样性有关。 关键词抗氧化剂;颜色;类黄酮;蜂蜜;Melipona beecheii。
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Polyphenol galangin induces the ROS and ER stress-mediated intrinsic and extrinsic apoptotic pathways towards human colon carcinoma HCT-116 cells
The Melipona beecheii honey has bioactive compounds with high antioxidant activity that influence its colour. This research evaluated the influence of phenolic compounds and flavonoids on the colour and antioxidant activity of M. beecheii honeys. Forty-three honey samples from different site located in the low deciduous forest of Yucatan, Mexico, were extracted during the harvest and post-harvest seasons (2020 – 2021). The Pfund method was used to measure colour, Folin-Ciocalteu method for phenols measurement, aluminum chloride method for flavonoids determination, and ABTS and DPPH IC50 assay to determine antioxidants. The predominant colour of the honey was extra light amber and light amber at both seasons. Phenols ranged from 780 to 1317 mg/kg GAE; flavonoids from 36 to 55 mg/kg CE and antioxidant were 675 to 1161 µmol/kg TEAC by the ABTS and 361.8 to 173.0 mg/mL by the DPPH IC50.  Phenols and flavonoids were correlated with colour and radical scavenging activity, indicating the influence of these compounds with the darker colour and higher antioxidant activity of honey. The statistically significant difference (P<0.05) between seasons could be associated with the botanical origin with great diversity of melliferous flora present in the low deciduous forest of the Yucatan Peninsula. Keywords: Antioxidant; colour; flavonoids; honey; Melipona beecheii.
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来源期刊
Emirates Journal of Food and Agriculture
Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY
CiteScore
1.80
自引率
0.00%
发文量
18
期刊介绍: The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.
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