比较橙提取物和石灰提取物对嗜水气单胞菌和嗜水气单胞菌的抗菌效果

IF 0.7 Q4 MICROBIOLOGY Journal of Pure and Applied Microbiology Pub Date : 2023-11-29 DOI:10.22207/jpam.17.4.38
N. D. A. Lubis, S. Amelia, Era Yusraini, Zikrina Rahmi, R. Balatif
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引用次数: 0

摘要

气单胞菌通常存在于水生环境中,可从各种鱼类中分离出来。在全球范围内,每 100 万人中感染气单胞菌的人数达到 0.6-76 例。橙子和酸橙一般用作烹饪香料,可以去除鱼的腥味,还具有抗菌作用。这项研究比较了石灰和橙子提取物对两种气单胞菌(A. sobria 和 A. hydrophila)的抗菌效果。在传统市场上购买的新鲜金枪鱼中可能会产生气单胞菌。石灰和橙子提取物的制备采用浸渍法,以 96% 的甲醇为溶剂。使用的提取物浓度分别为 6.25%、12.5%、25% 和 50%。采用 Kirby-Bauer 法进行了抗菌敏感性测试。细菌抑菌区测试表明,桔子和酸橙提取物对蚜虫 A. hydrophila 细菌的抑菌效果高于对梭状芽孢杆菌的抑菌效果。从抑菌区的平均直径可以看出,除了浓度为 6.25% 的桔子和 50% 的酸橙提取物外,其他提取物对蚜虫水虱的抑菌区都比对苏布里亚水虱的大。在提取物之间的比较中发现,石灰提取物的抗菌效果要好于橙提取物,但浓度为 6.5%的橙提取物和浓度为 50%的石灰提取物分别对苏布里亚氏菌和水飞蓟马进行了细菌试验。总的来说,石灰的抗菌效果要好于橘子。
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Comparison of Antimicrobial Effectiveness of Orange and Lime’s Extracts on Aeromonas sobria and Aeromonas hydrophila
Aeromonas bacteria are often found in aquatic environments and can be isolated from various types of fish. Globally, the incidence of infection by Aeromonas reached 0.6–76 cases per 1 million people. Orange and lime are generally used as cooking spices to get rid of the fishy smell in fish, and also have antibacterial effects. The study compared the antibacterial effectiveness of lime and orange extracts on two Aeromonas species; A. sobria and A. hydrophila. Bacteria Aeromonas may emerge from fresh tuna bought in traditional markets. Lime and orange extracts were prepared using the maceration method using 96% methanol as solvent. The extract concentrations used were 6.25%, 12.5%, 25%, and 50%. Antimicrobial sensitivity test was carried out using the Kirby-Bauer method. The bacterial inhibition zone test revealed that the effectiveness of extracts on both oranges and limes was higher for the bacteria A. hydrophila compared to A. sobria. It is known from the average diameter of the inhibition zone, which is larger on A. hydrophila compared to A. sobria, except for the extract with a concentration of 6.25% orange and 50% lime. In a comparison between extracts, lime extract was found to be more effective as an antimicrobial than orange extract, except at a concentration of 6.5% in the bacterial test A. sobria and 50% concentration in the bacterial test A. hydrophila. In general, lime is more effective as an antimicrobial than orange.
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来源期刊
Journal of Pure and Applied Microbiology
Journal of Pure and Applied Microbiology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
2.00
自引率
0.00%
发文量
266
审稿时长
11 months
期刊介绍: Journal of Pure and Applied Microbiology (JPAM) is a peer-reviewed, open access international journal of microbiology aims to advance and disseminate research among scientists, academics, clinicians and microbiologists around the world. JPAM publishes high-quality research in all aspects of microbiology in both online and print form on quarterly basis.
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