保护研究中的杂合度可视化方法综述

Andrey A. Tomarovsky, A. Totikov, Aliya R. Yakupova, A. Graphodatsky, Sergei F. Kliver
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摘要

估计杂合度水平是保护生物学的关键指标之一,因为它有助于正确设计濒危物种保护计划。随着全基因组测序技术的发展,现在不仅可以在生物体水平上,而且可以在种群和物种水平上更准确地估计杂合度。现代保护研究涉及到大量全基因组数据的处理,这就导致了解释方面的问题,因此有必要研究现代可视化方法,以便清晰、正确地展示结果。在这篇综述中,我们将详细介绍利用不同方法获得的杂合度水平估计值的主要可视化类型。我们详细介绍了每种可视化方法背后的理论,并以对不同保护状况的非模式物种的研究为例讨论了这些方法的特点。这篇综述让我们深入了解了当前估计杂合度并将其可视化的工具,以及该领域当前的发展趋势。
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Review of heterozygosity visualization approaches in the context of conservation research
Estimating heterozygosity levels is one of the key metrics in conservation biology, as it contributes to the correct design of conservation programs for endangered species. With the development of full-genome sequencing technologies, it is now possible to more accurately estimate heterozygosity not only at the organismal level, but also at the population and species level. Modern conservation studies involve the processing of large volumes of full-genomic data, which leads to problems of interpretation and necessitates the study of modern visualization methods for clear and correct presentation of results. In this review, we elaborate on the main types of visualization of heterozygosity level estimates obtained using different approaches. We detail the theory behind each visualization method and discuss their features using examples from studies of non-model species with different conservation status. The review provides insight into the current tools for estimating and subsequently visualizing heterozygosity, as well as current trends in the field.
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